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Millipore Anti-Dbf4 Antibody, Clone 7h8.1 Detects Level Of Dbf4 & Has Been Published & Validated For Use In Dbf4. - Mill (Additional S&H or Hazmat Fees May Apply)
DBF4, also known as Protein DBF4 homolog A, Activator of S phase kinase, also known as Chiffon homolog A or DBF4-type zinc finger containing protein 1, and encoded by the gene DBF4/ASK/DBF4A/ZDBF1, is the regulatory subunit for the cell cycle kinase CDC7. The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at Ser-40 and Ser-53 and then is involved in regulating the initiation of DNA replication during cell cycle. . DBF4 Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6 proteins. DBF4 is localized to the nucleus. DBF4 is expressed in expressed in many cells particularly high in testis, thymus and most cancer cells.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-DBF4 antibody, clone 7H8.1 detects level of DBF4 & has been published & validated for use in DBF4.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected DBF4 in 200 µg of human thymus tissue lysate.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human DBF4 near the C-terminus.
Epitope
Near C-terminus
Clone
7H8.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_006707
Gene Symbol
DBF4
ASK
DBF4A
ZDBF1
Purification Method
Protein G Purified
UniProt Number
Q9UBU7
Molecular Weight
~77 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected DBF4 in 200 µg of HepG2 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
