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Millipore This Anti-Mdm2 Antibody, Clone If2, Alexa Fluor® 488 Conjugate Is Validated For Use In Immunocytochemistry For The Detection Of Mdm2. - Mill (Additional S&H or Hazmat Fees May Apply)
E3 ubiquitin-protein ligase Mdm2 (UniProt Q00987; also known as Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2) is encoded by the MDM2 gene (Gene ID 4193) in human. MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes p53 transcriptional activity by binding to and blocking p53 N-terminal trans-activation domain. MDM2 can also monoubiquitinate p53, thereby promoting p53 cytoplasmic localization to prevent p53 nuclear funtion. In addition, MDM2, when in complex with p300, also mediates p53 polyubiquitination, resulting in p53 proteosomal degradation. MDM2 overexpression is shown to inhibit DNA double-strand break repair mediated through its interaction with Nbs1 in a p53-independent manner.
Product Information
Format
AlexaFluor®488
Presentation
Purified mouse monoclonal IgG2b antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of MDM2.
Key Applications
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MDM2 in A431 cells. The unconjugated antibody (Cat. Nos. MABE340 & OP46) is shown to be suitable also for immunohistochemistry and Western blotting applications.
Biological Information
Immunogen
Recombinant human MDM2.
Epitope
Within amino acids 26-169.
Clone
IF2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone IF2 recognizes the ~85/90 kDa (apparent MW) MDM2, as well as isoforms of ~57/60 kDa and ~74/76 kDa by immunoblotting using the unconjugated antibody (Cat. Nos. MABE340 & OP46).
Isotype
IgG2b
Species Reactivity
Human
Species Reactivity Note
Human.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002383
Gene Symbol
MDM2
Purification Method
Protein g purified.
UniProt Number
Q00987
Molecular Weight
~85 kDa observed.
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MDM2 in HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
