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Millipore This Anti-Phospho Histone H2b (Tyr37) Antibody, Clone 15 Is Validated For Use In Western Blotting & Chip For The Detection Of Phospho Histone H2b (Tyr37). - Mill (Additional S&H or Hazmat Fees May Apply)
Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the beads on a string structure.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-phospho Histone H2B (Tyr37) antibody, clone 15 is validated for use in western blotting & ChIP for the detection of phospho Histone H2B (Tyr37).
Key Applications
Western Blotting
Chromatin Immunoprecipitation (ChIP)
Application Notes
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho Histone H2B (Tyr37) in mouse MEF cells which were untreated or treated with WEE inhibitor (Mahajan, K., et al. (2012). Nat Struct Mol Biol. 19(9):930-937.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho Histone H2B (Tyr37) in MEF-overexpressing WEE (Mahajan, K., et al. (2012). Nat Struct Mol Biol. 19(9):930-937.).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H2B phosphorylated at Tyr37.
Clone
15
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_778225
Gene Symbol
HIST3H2BB
Purification Method
Protein G Purified
UniProt Number
Q8N257
Molecular Weight
~17 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2B (Tyr37) in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
