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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B, Xn | WB, ICC, IHC, FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE446 |
| Description | Anti-Histone H1° Antibody, clone 34 |
| Alternate Names |
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| Background Information | Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Histone H1° Antibody, clone 34 is a highly specific mouse monoclonal antibody, that targets Histone H1 & has been tested in western blotting, ICC, IHC & Flow Cytometry. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H1° in Xenopus embryo tissue lysate (Seigneurin, D., et al. (1995). Int J Dev Biol. 39(4):597-603.; Fu, G., et al. (2003). Biol Reprod. 68(5):1569-1576.). Immunocytochemistry Analyis: A representative lot from an independent laboratory detected Histone H1° in Xenopus unfertilized eggs and early embryos (Fu, G., et al. (2003). Biol Reprod. 68(5):1569-1576.; Adenot, P. G., et al. (2000). J Cell Sci. 113(Pt 16):2897-2907.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Histone H1° in Xenopus embryo tissues (Grunwald, D., et al. (1995). Exp Cell Res. 218(2):586-595.). Flow Cytometry Analyisis: A representative lot from an independent laboratory detected Histone H1° in FC (Grunwald, D., et al. (1999). Methods Mol Biol. 119:443-454.). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to Ox liver Histone H1° |
| Clone | 34 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes Histone H1°. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with Human, Mouse, Rat, Bovine, and Xenopus. Predicted to react with Ox based upon immunogen design |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~30 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H1° in 10 µg of Jurkat cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |