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Millipore Detect Tet2 Using This Mouse Monoclonal Antibody, Anti-Tet2 Antibody, Clone Ht2h 21f11 Validated For Use In Chromatin Ip (Chip), Western Blotting & Ip. - Mill (Additional S&H or Hazmat Fees May Apply)
Methylcytosine dioxygenase TET2 catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The function of 5-hydroxymethylcytosine is currently unclear but it may influence chromatin structure, or act as an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD) or myeloproliferative neoplasms (MPN), and systemic mastocytosis. TET2 disorders also cause polycythemia vera (PV) and myelodysplastic syndrome (MDS).
Product Information
Format
Purified
Control
MOLT-4 cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Tet2 using this mouse monoclonal antibody, Anti-TET2 Antibody, clone hT2H 21F11 validated for use in Chromatin IP (ChIP), western blotting & IP.
Key Applications
Chromatin Immunoprecipitation (ChIP)
Western Blotting
Immunoprecipitation
Application Notes
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected TET2 in 10 µg of TF-1 cell lysate. Immunoprecipitation Analysis: 5 µg of a representative lot immunoprecipitated TET2 in 250 µg of HL60 RIPA cell lysate.
Biological Information
Immunogen
Recombinant protein corresponding to the N-terminus of human TET2.
Epitope
N-terminus
Clone
hT2H21F11
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes the N-terminus of TET2
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001120680
Gene Symbol
TET2
Purification Method
Protein G Purified
UniProt Number
Q6N021
Molecular Weight
~ 260 kDa observed. The calculated molecular weight of this protein is 224 kDa, but can be observed at ~260 kDa.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in MOLT-4 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected TET2 in 10 µg of MOLT-4 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
