Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE664-25UG |
| Description | Anti-CBX1 (HP1 beta) Antibody, clone 5E9.1 |
| Alternate Names |
|
| Background Information | Chromobox protein homolog 1 (UniProt: P83916; also known as Heterochromatin protein 1 homolog beta, HP1 Hsbeta, HP1 beta, Heterochromatin protein p25, M31, Modifier 1 protein, p25 beta) is encoded by the Cbx1 (also known as Cbx) gene (Gene ID: 12412) in human. Chromobox protein homolog 1 is a homodimeric nuclear protein that is a component of heterochromatin. It is expressed in all adult and embryonic tissues. It is essential for formation of and gene repression in heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Also interacts with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. The human HP1 homolog CBX1 preferentially recognize H3K9me3 (Peptide binding specificity Kd = 5 μM). However, upon substitution of Threonine 6 to Alanine reduces its affinity significantly (Kd = 45 mm). (Ref.: Kaustov, L., et Al. (2011). J. Biol. Chem. 286(1): 521-529). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-CBX1 (HP1 beta), clone 5E9.1 Antibody, Cat. No. MABE664-25UG, is a highly specific mouse monoclonal antibody, that targets CBX1 and has been tested in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant protein fragment corresponding to 60 amino acids from the internal region of human Chromobox protein homolog 1 (CBX1). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5E9.1 specifically detects human Chromobox protein homolog 1 (CBX1). It targets an epitope with in 60 amino acids from the internal region. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
|
| Molecular Weight | 23 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected CBX1 (HP1 beta) in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |