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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, ChIP | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE872 |
| Description | Anti-Histone H3.3 Antibody, clone 4H2D7 |
| Alternate Names |
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| Background Information | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Histone H3.3 Antibody, clone 4H2D7 is validated for use in western blotting, ICC & ChIP for the detection of Histone H3.3. |
| Key Applications |
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| Application Notes | Immunocytochemistry Analysis: A 1:50 dilution from a represenative lot detected Histone H3.3 in HeLa cells. Western Blotting Analysis: A representative lot from an independent laboratory detected recombinant Histone H3.3 (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.). Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.). Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.3 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Histone H3.3. |
| Clone | 4H2D7 |
| Concentration | 1 mg/mL |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~15 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa acid extract. Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |