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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE878 |
| Description | Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 |
| Alternate Names |
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| Background Information | RNA Helicase A, also known as DEAH box protein 9 (mHEL-5), DHX9, or Nuclear DNA helicase II (NDH II), and encoded by the mouse gene Dhx9, is a component of the CRD-mediated mRNA stability complex. The CRD-mediated complex is a protein complex that binds to, and promotes stabilization of, mRNA molecules containing the coding region instability determinant (CRD). In humans, it consists of IGF2BP1 and at least four additional proteins: HNRNPU, SYNCRIP, YBX1, and RNA Helicase A. RNA Helicase A operates by unwinding dsDNA and RNA in a 3 to 5 direction. Additionally, RNA Helicase A acts as a transcriptional activator and interacts with LARP6 which is a protein that has been associated with fasting glucose traits, type 2 diabetes and obesity as well as the stabilization of collagen mRNAs for CO1A1 and CO1A2. RNA Helicase A is localized to the nucleolus within the nucleus but can also be found in the cytoplasm localized in cytoplasmic mRNP granules containing untranslated mRNAs and thus the protein shuttles between the nucleus and cytoplasm depending upon the microenvironment. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 is validated for use in western blotting & ICC for the detection of RNA Helicase A/Dhx9. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cell lysate (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261). Immunocytochemistry Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cells (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminus of mouse RNA Helicase A/Dhx9. |
| Epitope | N-terminus |
| Clone | 8E3 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~140 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa nuclear extract. Western Blotting Analysis: 0.5 µg/mL of this antibody detected RNA Helicase A/Dhx9 in 10 µg of HeLa nuclear extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |