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The protein named U6 snRNA-associated Sm-like protein LSm1 or Cancer associated Sm-like, or Small nuclear ribonuclear CaSm and encoded by the human gene LSM1/CASM is a critical binding protein that mediates replication dependent histone mRNA degradation during cell division. Lsm1 is part of the Lsm family of proteins. Lsm proteins are a ubiquitous family of proteins characterized by the Sm-domain. They exist as hexa- or heptameric RNA-binding complexes and carry out RNA-related functions. Lsm1 interacts directly with SLBP a histone stem-loop binding protein critical for histone mRNA processing and expression. Histone mRNAs do not contain introns or polyadenylation signals and are processed by direct endonucleolytic cleavage. Lsm1 binds directly to the 3’-terminal U-tract of U6 snRNA . Lsm1 is localized to the nucleus and is up regulated in many cancers including pancreatic cancer and many tumor cell lines.
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-LSm1 Antibody, clone 5F3 is an antibody against LSm1 for use in western blotting & IP.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Western Blotting Analysis: A representative lot from an independent laboratory detected LSm1 in MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated LSm1 from MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).
Biological Information
Immunogen
Recombinant protein corresponding to mouse LSm1.
Clone
5F3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rat
Isotype
IgG1
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_080308
Gene Symbol
Lsm1
Purification Method
Protein G Purified
UniProt Number
Q8VC85
Molecular Weight
~15 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in L-929 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected LSm1 in 10 µg of L-929 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
