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Millipore This Anti-Asf-1 Antibody, Clone Ak-96 Is Validated For Use In Western Blotting, Ip & Icc For The Detection Of Asf-1. - Mill (Additional S&H or Hazmat Fees May Apply)
Alternative-splicing factor 1 (ASF-1), also known as SRp30a or SF2, is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-ASF-1 Antibody, clone AK-96 is validated for use in western blotting, IP & ICC for the detection of ASF-1.
Key Applications
Western Blotting
Immunoprecipitation
Immunocytochemistry
Application Notes
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of Raji and HepG2 cell lysate. Immunocytochemistry Analysis: A representative lot detected ASF-1 in BJ fibroblast cells (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66). Immunoprecipitation Analysis: A representative lot immunoprecipitated ASF-1 in BJ fibroblast cell lysate (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66).
Biological Information
Immunogen
Recombinant protein corresponding to human ASF-1.
Clone
AK-96
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001071634
Gene Symbol
SRSF1
ASF
SF2
SF2P33
SFRS1
Purification Method
Protein G Purified
UniProt Number
Q07955
Molecular Weight
~30 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
