The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore This Anti-Mif Antibody, Clone M1, Preservative Free Is Validated For Use In Flow Cytometry, Neutralizing & Western Blotting For The Detection Of Mif. - Mill (Additional S&H or Hazmat Fees May Apply)
MIF, also known as Macrophage migration inhibitory factor, or Glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, L-dopachrome tautomerase, or Phenylpyruvate tautomerase, and encoded by the gene MIF/GLIF/MMIF, is proinflammatory cytokine involved in the innate immune response to bacterial pathogens. MIF is secreted by macrophages upon stimulation by bacterial lipopolysaccharide or bacterial antigens, and the expression of MIF at sites of inflammation suggests a role as a mediator in regulating the function of macrophages in host defense. MIF also counteracts the anti-inflammatory activity of glucocorticoids and has various enzyme activities but their physiological function is not known. MIF expression is primarily lymphocytes, macrophages and immune system cells. Defects in MIF may be associated with Rheumatoid arthritis systemic juvenile disease, an inflammatory articular disorder with systemic onset beginning at age 16. Also serum levels of MIF are elevated in cases of severe sepsis and are correlated with low survival.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Quality Level
MQ100
Applications
Application
This Anti-MIF Antibody, clone M1, preservative free is validated for use in flow cytometry, neutralizing & western blotting for the detection of MIF.
Key Applications
Flow Cytometry
Neutralizing
Western Blotting
Application Notes
Neutralization Assay Analysis: A representative lot from an independent laboratory detected MIF during the migration of human peripheral blood granulocytes (Rupreht, R. R., et al. (2000). Pflugers Arch. 440(5 Suppl):R78-80.).
Biological Information
Immunogen
Recombinant protein corresponding to human MIF.
Clone
M1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002406
Gene Symbol
MIF
GLIF
MMIF
Purification Method
Protein G Purified
UniProt Number
P14174
Molecular Weight
12 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Flow Cytometry by Jurkat cells.
Flow Cytometry: 1 µg of this antibody detected MIF in 1X10E6 Jurkat cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
