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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF18 |
| Replaces | 04-462; MABF22 |
| Description | Anti-Interleukin-1 beta (IL-1B) Antibody, clone 4A7 |
| Alternate Names |
|
| Background Information | Interleukin-1 (IL-1) is a cytokine that is thought to be connected to neurodegeneration and in central nervous system-mediated defense reactions to inflammation. Interleukin-1 beta (IL-1B) maintains mitogen-induced proliferation, IL-2 production and expression of IL-2R. IL-1 beta also seems to have the ability to boost the number of cells that move into the G1 phase of the cell cycle. IL-1 beta also has the capacity to stimulate apoptosis on normal human fetal membranes. IL-1 beta may play an important role in inflammation regulating cytokine networks. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Interleukin-1 beta (IL-1B) Antibody, clone 4A7 is an antibody against Interleukin-1 beta (IL-1B) for use in WB. |
| Key Applications |
|
| Application Notes | Luminex Assay Analysis: A previous lot was used by an independent laboratory in Luminex (Dr. Gerard Zurawski, Baylor Institute for Immunology Research, Dallas, TX). |
| Biological Information | |
|---|---|
| Immunogen | Full length recombinant protein corresponding to human Interleukin-1 beta. |
| Clone | AB34_41.4H12.4A7 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. SUBUNIT STRUCTURE: Monomer. SUBCELLULAR LOCATION: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. DOMAIN: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. SEQUENCE SIMILARITIES: Belongs to the IL-1 family. |
| Molecular Weight | ~17 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in lysate from cells expressing human Interleukin-1 beta recombinant protein. Western Blot Analysis: 0.5 µg/mL of this antibody detected 10 µg of human Interleukin-1 beta recombinant protein. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |