Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | FC, IHC, WB, FUNC, IP | M | Affinity Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF1970-25UL |
| Description | Anti-NKp46 (CD335) Antibody, clone WEN23 |
| Alternate Names |
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| Background Information | Natural cytotoxicity triggering receptor 1 (UniProt: Q9Z0H5; also known as Activating receptor 1, rAR-1, Lymphocyte antigen 94 homolog, NK receptor KILR-1, NKACTR, Natural killer cell p46-related protein, NK-p46, NKp46, CD335) is encoded by the Ncr1 (also known as Ar1, Kilr1, Ly94) gene (Gene ID: 117547) in rat. NK-p46 is a single-pass type I membrane protein that belongs to the natural cytotoxicity receptor (NCR) family and serves as a NK-p46 is an activating NK cell receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NK-p46 also regulates graft-versus-host disease and allergic response. Following the initiation of an NK-target cell interaction, NK-p46 is reported to cluster at the cell membrane, specifically at the immune synapse where it mediates cytoskeletal rearrangement and cellular polarization. NK-p46 has an extracellular domain (aa 17-258), a helical domain (aa 259-279), and a cytoplasmic domain (aa 280-325). It also has two Ig-like domains (aa 42-100 and 137-192). It is synthesized with a signal peptide of 16 amino acids, which is subsequently cleaved off. NK-p46 is expressed by all natural killer (NK) cells, but not by T cells, B cells, granulocytes, monocytes, dendritic cells, or macrophages. It is weakly expressed in spleen, heart, and lung tissue. The main NK cell population of the lymph node expresses low levels of NK-p46 in resting cells, however, its expression is elevated by the presence of interleukin-2. (Ref.: Hadad, U et Al. (2015). Front. Immunol 60 (Article 495); 1-11). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-NKp46 (CD335), clone WEN23 Antibody, Cat. No. MABF1970-25UL is validated for use in Flow Cytometry and Immunohistochemistry, Western Blotting, Affects Function and Immunoprecipitation, for the detection of Natural cytotoxicity triggering receptor 1 . |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected NKp46 (CD335) in Western Blotting applications (Westgaard, I.H., et. Al. (2004). J Leukoc Biol. 76(6):1200-6). Affects Function Analysis: A representative lot of anti-NKp46 (CD335) affected funtion of NKp46 (CD335). (Westgaard, I.H., et. Al. (2004). J Leukoc Biol. 76(6):1200-6). Immunoprecipitation Analysis: A representative lot detected NKp46 (CD335) in Immunoprecipitation applications (Westgaard, I.H., et. Al. (2004). J Leukoc Biol. 76(6):1200-6). Flow Cytometry Analysis: A representative lot detected NKp46 (CD335) in Flow Cytometry applications (Westgaard, I.H., et. Al. (2004). J Leukoc Biol. 76(6):1200-6). Immunohistochemistry Analysis: A representative lot detected NKp46 (CD335) in Immunohistochemistry applications (Smelt, M.J., et. Al. (2014). Cell Transplant. 23(11):1381-94). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant rat NKp46 ectodomain coupled to the Fc portion of mouse IgG2b. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone WEN23 detects natural cytotoxicity receptor 1 (NCR1, NK-p46) in rat. It targets an epitope in the ectodomain regioin. |
| Isotype | IgG1 |
| Species Reactivity |
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| Species Reactivity Note | Rat. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 37 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in HEK293 cells transfected with rat NKp46. Flow Cytometry Analysis: 1 µg of this antibody detected NKp46 (CD335) in HEK293 cells transfected with rat NKp46. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |