Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | NEUT, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF1973-25UL |
| Description | Anti-Etanercept Antibody, clone ETA63C8 |
| Alternate Names |
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| Background Information | Etanercept is a chimeric fusion protein consisting of the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1. This Fc component contains the hinge, CH2 and CH3 regions, but not the CH1 region of IgG1. Etanercept binds to TNF-alpha and blocks its interactions with receptors. Hence, it is considered a TNF inhibitor that functions as a decoy receptor that binds to TNF. Although it mimics the inhibitory effects of naturally occurring soluble TNF receptors, the difference is that Etanercept has a greatly extended half-life in the bloodstream. Its speci fic activity is reported to be about 1.7 x 106 units/mg. Etanercept is shown to modulate biological responses produced by TNF, including expression of adhesion molecules responsible for leukocyte migration, serum levels of cytokines, and serum levels of matrix metalloproteinase-3. It is also shown to affect animal models of inflammation, including murine collagen-induced arthritis. Clone ETA63C8 can bind to Etanercept and block its activity. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Etanercept, clone ETA63C8 Antibody, Cat. No. MABF1973-25UL is validated for use in Neutralizing and ELISA, for the detection of Etanercept. |
| Key Applications |
|
| Application Notes | Neutralizing Analysis: A representative lot neutralized Etanercept in Neutralizing applications (Detrez, I., et. Al. (2016). J Immunol. 196(6):2879-84). ELISA Analysis: A representative lot detected Etanercept in ELISA applications (Detrez, I., et. Al. (2016). J Immunol. 196(6):2879-84). ELISA Analysis: 4 µg/mL from a representative lot detected Etanercept in human serum with AS (ankylosing spondylitis) (Courtesy of Professor Ann Gils, pH.D., KU Leuven Belgium). |
| Biological Information | |
|---|---|
| Immunogen | Chimeric fusion protein cotaining the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1. |
| Clone | ETA63C8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone ETA63C8 detects Etanercept in human samples. It can bind to Etanercept and block its activity. |
| Isotype | IgG1 |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein g purified |
| Molecular Weight | 150 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Isotype testing: Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |