Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | INHIB, ACT, FC, ICC, NEUT | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF1976 |
| Description | Anti-Complement Receptor C5aR Antibody, clone 20/70 |
| Alternate Names |
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| Background Information | C5a anaphylatoxin chemotactic receptor 1 (UniProt: P30993; also known as C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88) is encoded by the C5ar1 (also known as C5ar, C5r1) gene (Gene ID: 12273) in murine species. C5aR is a multi-pass membrane protein of the g-protein coupled receptor 1 family that serves as a receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. C5aR is a homodimer, but can also form higher-order oligomers. C5aR becomes phosphorylated on serine residues in response to C5a binding that results in internalization of the receptor and short-term desensitization to C5a. The phosphorylated receptor is known to Interact with arrestin beta 1 and 2 (ARRB1 and 2) and this interaction is associated with internalization of C5aR. Sulfation is also reported to play a critical role in the association of C5aR with C5a, but it does not alter the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2b in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Complement Receptor C5aR, clone 20/70, Cat. No. MABF1976, is a rat monoclonal antibody that detects Complement 5a receptor (C5aR) and has been tested for use in Flow Cytometry, Activity Assay, Inhibition of Activity/Function, Immunocytochemistry, and Neutralizing. |
| Key Applications |
|
| Application Notes | Neutralizing Analysis: A representative lot neutralized Complement receptor C5aR (Godau, J., et. Al. (2004). J Immunol. 173(5):3437-45). Immunocytochemistry Analysis: A representative lot detected Complement receptor C5aR in Immunocytochemistry applications (Soruri, A., et. Al. (2003). Immunol Lett. 88(1):47-52). Inhibits Activity/Function Analysis: A representative lot inhibited Complement receptor C5aR in Activity/Function applications (Godau, J., et. Al. (2004). J Immunol. 173(5):3437-45; Soruri, A., et. Al. (2003). Flow Cytometry Analysis: A representative lot detected Complement receptor C5aR in Flow Cytometry applications (Shushakova, N., et. Al. (2002). J Clin Invest. 110(12):1823-30). Activity Assay Analysis: A representative lot detected Complement receptor C5aR in Activity Assay applications (Shushakova, N., et. Al. (2002). J Clin Invest. 110(12):1823-30). |
| Biological Information | |
|---|---|
| Immunogen | Rat basophilic leukemia RBL-2H3 cells stably expressing murine C5aR. |
| Epitope | extracellular domain |
| Clone | 20/70 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 20/70 detects complement 5a receptor in murine species. It targets an epitope with in the extracellular domain. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 39.02 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in BALB/c mouse bone marrow cells. Flow Cytometry Analysis: 1 µg of this antibody detected Complement receptor C5aR in 1 million BALB/c mouse bone marrow cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |