| Description | |
|---|---|
| Catalogue Number | MABF1997-25UG |
| Description | Anti-Lap2alpha Antibody, clone 8C10-1H11 |
| Alternate Names |
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| Background Information | Lamina-associated polypeptide 2, isoforms alpha (UniProt: Q61033; also known as Thymopoietin isoform alpha, TP alpha) is encoded by the Tmpo (also known as Lap2) gene (Gene ID: 21917) in murine species. LAP2alpha is a non-membrane bound, oligomeric nucleoplasmic protein that associates directly or indirectly with lamins at specific cell-cycle stages. It is expressed diffusely throughout the nucleus and is involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. It is shown to interact with A-type lamins to control gene expression, transcription, and chromatin organization. The N-terminal part of LAP2alpha contains two structurally independent, non-interacting domains: LEM (LAPEmerin-MAN1)-like domain that binds DNA and a LEM domain that interacts with BANF1. Its C-terminal domain forms a four-stranded coiled coil. Lamin A/C and LAP2alpha complexes predominantly co-occur in euchromatic genomic regions and are shown to be highly mobile. They can bind chromatin in a dynamic manner and are involved in establishing a chromatin environment that is permissive for epigenetic regulation. Loss of LAP2alpha has been linked to increased proliferation of tissue progenitor cells in epidermis, colon, and the hematopoietic system and delayed skeletal muscle differentiation. (Res.: Gesson, K et Al., (2016). Genome Res. 26(4);462-473). |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-Lap2alpha, clone 8C10-1H11, Cat. No. MABF1997, is a mouse monoclonal antibody that detects Lamina-associated polypeptide 2, isoforms alpha, and has been tested for use in Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), and Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A representative lot detected Lap2alpha in Western Blotting applications (Gesson, K., et. Al. (2016). Genome Res. 26(4):462-73). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Lap2alpha in Chromatin Immunoprecipitation applications (Gesson, K., et. Al. (2016). Genome Res. 26(4):462-73). Immunoprecipitation Analysis: A representative lot immunoprecipitated Lap2alpha in Immunoprecipitation applications (Gesson, K., et. Al. (2016). Genome Res. 26(4):462-73). Immunofluorescence Analysis: A representative lot detected Lap2alpha in Immunofluorescence applications (Gesson, K., et. Al. (2016). Genome Res. 26(4):462-73). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment corresponding to 507 amino acids from the C-terminal region. |
| Epitope | cytoplasmic domain |
| Clone | 8C10-1H11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 8C10-1H11 specifically detects Lamina-associated polypeptide 2 alpha (LAP2alpha) in murine species. It targets an epitope in the internal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~75 kDa observed; 75.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in Knockout (KO) vs. wild-type (WT) mouse fibroblast cell lysates. Western Blotting Analysis: 1 µg/mL of this antibody detected Lamina-associated polypeptide 2, isoforms alpha (LAP2alpha) in wild-type, but not in Knockout (KO) mouse fibroblast cell lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |