Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2003-100UG |
| Description | Anti-CD11a Antibody, clone BU17 |
| Alternate Names |
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| Background Information | Integrin alpha-L (UniProt: P20701; also known as CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain, CD11a) is encoded by the ITGAL (also known as CD11A) gene (Gene ID: 3683) in human. Integrins are part of a family of heterodimeric transmembrane glycoproteins that recognize a variety of ligands and control numerous physiologic functions in cells. CD11a is a single-pass type I membrane protein that noncovalently associates with beta2 integrin to forms a heterodimer and serve as a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils, and eosinophils. It is also involved in generation of common lymphoid progenitor cells in bone marrow. In resting T-cells, about 40% of CD11a is constitutively phosphorylated and phosphorylation causes conformational changes needed for ligand binding and is considered to be essential for activation by some physiological agents. CD11a is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off in the mature form. It has a large extracellular domain (aa 26-1090), a transmembrane domain (aa 1091-1111), and a cytoplasmic domain 1112-1170). Three isoforms of CD11a have been described that are produced by alternative splicing. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-CD11a, clone BU17, Cat. No. MABF2003, is a mouse monoclonal antibody that detects CD11a and has been tested for use in Flow Cytometry. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected CD11a in Flow Cytometry applications (Baker, M.P., et. Al. (1998). Blood. 92(8):2830-43). |
| Biological Information | |
|---|---|
| Immunogen | Waldenstrom macroglobulinemia lymphoplasmacytic cells. |
| Epitope | extracellular domain |
| Clone | BU17 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone BU17 detects CD11a in human cells. It targets an epitope with in the extracellular domain. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 128.77 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in one million human peripheral blood mononuclear cells. Flow Cytometry Analysis: 1 µg of this antibody detected CD11a in one million human peripheral blood mononuclear cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |