| Description | |
|---|---|
| Catalogue Number | MABF2005-25UG |
| Description | Anti-CCR5 (CD195) Antibody, clone HEK/1/85a/7a |
| Alternate Names |
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| Background Information | C-C chemokine receptor type 5 (UniProt: P51681; also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195) is encoded by the CCR5 (also known as CMKBR5) gene (Gene ID: 1234) in human. CCR-5 is a multi-pass membrane protein that is expressed in memory T-cells, macrophages, and immature dendritic cells, and is upregulated by proinflammatory cytokines. It is also shown to be highly expressed in spleen, thymus, and on THP-1 and kg-1a cells. Lower expression is observed in ovary and lung. CCR-5 serves as a receptor for a number of inflammatory CC-chemokines and subsequently transduces a signal by increasing intracellular calcium levels. It also plays a role in the control of granulocytic lineage proliferation or differentiation. CCR-5 is acts as a co-receptor (CD4 being the primary receptor) of HIV-1 and HIV-2. It also couples to Gi class of g-proteins to inhibit cAMP production and stimulate calcium release. CCR-5 undergoes sulfation on two tyrosine residues in the N-terminal region, which increases efficiency of HIV-1 entry into cells. It also undergoes phosphorylation at C-terminal serine residues following binding to CC chemokines, especially APO-RANTES. Mutations in CCR5 gene have been linked to insulin-dependent diabetes mellitus. (Ref.: Blanpain, C., et Al. (2002). Receptors Channels. 8(1);19-31). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-CCR5 (CD195), clone HEK/1/85a/7a, Cat. No. MABF2005, is a rat monoclonal antibody that detects C-C chemokine receptor type 5 (CCR5) and has been tested for use in Flow Cytometry and Immunocytochemistry. |
| Key Applications |
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| Application Notes | Immunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. Al. (2002). Blood. 99(3):785-91). Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. Al. (2002). Blood. 99(3):785-91). |
| Biological Information | |
|---|---|
| Immunogen | CHO Cells transfected with full length human CCR-5 (CD195). |
| Epitope | extracellular domain |
| Clone | HEK/1/85a/7a |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195). |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 40.52 kDa calculated. |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Flow Cytometry in wrote RBL-2H3 cells stably transfection with CCR5. Flow Cytometry Analysis (FC): 1 µg of this antibody detected C-C chemokine receptor type 5 (CD195) in one million CCR-5 transfected RBL-2H3 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
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| Material Size | 25 μg |