Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Virus | IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2038-25UG |
| Description | Anti-Rabies virus Antibody, clone 802 |
| Alternate Names |
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| Background Information | Glycoprotein (UniProt: P03524) of Rabies virus of Evelyn-Rokitnicki-Abelseth (ERA) strain is encoded by the g gene. Rabies virus belongs to the Lyssavirus serogroup of viruses. The glycoprotein of Rabies virus is a homotrimer, single-pass type I membrane protein that attaches the virus to host cellular receptor and thereby inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. It has been shown that the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and facilitate Rabies virus entry into cells. Arginine 352 is considered to be highly involved in Rabies virus pathogenicity and a mutation at this site dramatically attenuates the virus. Rabies virus glycoprotein is synthesized with a signal peptide (aa 1-19) that is subsequently cleaved off. It has two topological domains (aa 20-459 virion surface; 481-524 intravirion) and a helical domain (aa 460-480). Glycosylation of viral glycoprotein is considered to be crucial for glycoprotein export at the cell surface. Clone 802 is shown to recognize most Rabies virus strains, however it does not display neutralizing activity. (Ref.: Schumacher, CL et Al. (1989). J. Clin. Invest. 84(3); 971-975; Bunschoten, H et Al. (1989). J. Gen. Virol. 70(2); 291-298). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in PBS without preservatives. |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Rabies virus, clone 802, Cat. No. MABF2038, is a mouse monoclonal antibody that detects glycoprotein of Rabies virus and has been tested for use in Immunohistochemistry. |
| Key Applications |
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| Application Notes | Immunohistochemistry Analysis: A representative lot detected Rabies virus in CVS-24 infected mouse and normal mouse tissues (Courtesy of the Ertl Lab, Wistar Institute, Philadelphia, PA USA). |
| Biological Information | |
|---|---|
| Immunogen | Beta-propiolactone-inactivated Rabies Virus (ERA strain). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 802 detects Glycoprotein of Rabies virus (strain ERA). This clone does not display neutralizing activity in vitro. |
| Isotype | IgG2b |
| Species Reactivity |
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| Species Reactivity Note | Rabies Virus. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 58.6 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Isotype testing: Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |