Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2044 |
| Description | Anti-MCPyV large T-antigen Antibody, clone CM2B4 |
| Alternate Names |
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| Background Information | Merkel cells are neuroendocrine cells found in skin that have synaptic contacts with somatosensory afferents. These cells can turn malignant and form an aggressive form of skin cancer, which is known as Merkel cell carcinoma (MCC). A vast majority of MCC are caused by a polyomavirus known as Merkel cell polyomavirus (MCPyV or MCV). The MCPyV genome is reported to undergo clonal integration into the host cell chromosomes of MCC tumors and expresses small T antigen and truncated large T antigen. Full-length MCPyV large T-antigen is a 125 kDa nuclear protein, however, MCPyV T-antigens obtained from MCC have natural truncating mutations, which result in variably-sized, smaller proteins. Based on homology to other polyomaviruses, the MCPyV large and small T antigens are predicted to be oncogenic and contribute directly to the carcinogenesis of MCC. MCPyV large T antigen can serve as a specific marker for MCC. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MCPyV large T-antigen, clone CM2B4, Cat. No. MABF2044, is a mouse monoclonal antibody that detects MCPyV large T antigen and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting for the detection of Merkel cell polyomavirus. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected MCPyV large T-antigen in Western Blotting applications (Liu, X., et. Al. (2011). J Biol Chem. 286(19):17079-90; Rodig, S.J., et. Al. (2012) J Clin Invest. 122(12):4645-53). Immunoprecipitation Analysis: A representative lot detected MCPyV large T-antigen in Immunoprecipitation applications (Liu, X., et. Al. (2011). J Biol Chem. 286(19):17079-90). Immunohistochemistry Analysis: A representative lot detected MCPyV large T-antigen in Immunohistochemistry applications (Rodig, S.J., et. Al. (2012) J Clin Invest. 122(12):4645-53; Busam, K.J., et. Al. (2009). Am J Surg Pathol. 33(9):1378-85). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to RSRKPSSNASRGA sequence from the Merkel cell polyomavirus large T antigen exon 2 with a C-terminal cystiene. |
| Clone | CM2B4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone CM2B4 is highly specific for MCPyV large T and 57kT isoforms, but does not detect MCPyV small T antigen. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein g purified |
| Molecular Weight | 45 kDa observed for truncaed form. For intact form MW 125 kDaUncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MKL-1 cell lysates. Western Blotting Analysis: 1 µg/mL of this antibody detected MCPyV large T-antigen in 10 µg of MKL-1 cell lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |