| Description | |
|---|---|
| Catalogue Number | MABF2047-25UG |
| Description | Anti-BST-2 (CD317) Antibody, clone 927 |
| Alternate Names |
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| Background Information | Bone marrow stromal antigen 2 (UniProt: Q8R2Q8; also known as BST-2, HM1.24 antigen, PDCA-1, CD317) is encoded by the Bst2 gene (Gene ID: 69550) in murine species. BST-2 is a single-pass type II disulfide-linked homodimeric membrane protein that shuttles between the cell membrane, where it is present predominantly in membrane/lipid rafts, and the trans-Golgi network. Its dimerization is essential for its antiviral activity. BST-2 is also shown to form a complex with MMP-14 and localize to the cytoplasm. BST-2 is highly expressed on type I interferon-producing cells in naïve mice. Its levels in most cells are elevated following treatment with type I IFNs and IFN-gamma. It serves as an IFN-induced antiviral host restriction factor that efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. It acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be either internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. BST-2 is synthesized with a propeptide (aa 153-172), which is removed to generate the mature form. The mature form has a cytoplasmic domain (aa 1-30), a transmembrane domain (aa 31-51), and the extracellular domain (aa 52-152). Within the extracellular region, it has a coiled coil domain (aa 74-147) that forms an extended 17 nm long semi-flexible rod-like structure, which is essential for virion retention at the cell surface and prevent virus spreading. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2b in PBS without azide. |
| Applications | |
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| Application | Anti-BST-2 (CD317), clone 927, Cat. No. MABF2047, is a rat monoclonal antibody that detects Bone marrow stromal antigen 2 and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Neutralizing activity. |
| Key Applications |
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| Application Notes | Neutralizing Analysis: A representative lot efficiently depleted type I interferon-producing cells (IPC) and abrogated IFN-alpha secretion by IPC in response to CpG. (Blasius, A.L., et. Al. (2006). J Immunol. 177(5):3260-5). Immunohistochemistry (Paraffin) Analysis: A representative lot detected BST-2 (CD317) in Immunohistochemistry applications (Yun, T., et. Al. (2016). Cell Metab. 23(5):852-66). ELISA Analysis: A representative lot detected BST-2 (CD317) in ELISA applications (Blasius, A.L., et. Al. (2006). J Immunol. 177(5):3260-5). Flow Cytometry Analysis: A representative lot detected BST-2 (CD317) in Flow Cytometry applications (Blasius, A.L., et. Al. (2006). J Immunol. 177(5):3260-5; Yun, T., et. Al. (2016). Cell Metab. 23(5):852-66). |
| Biological Information | |
|---|---|
| Immunogen | Type I IFN-producing cells derived from mouse bone marrow. |
| Epitope | extracellular domain |
| Clone | 927 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 927 is a rat monoclonal antibody that specifically detects murine Bone marrow stromal antigen 2 (BST-2). It targets an epitope with in the extracellular domain. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~19.15 kDa calculated. |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in mouse spleen and mouse kidney tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected BST-2 (CD317) in mouse spleen and mouse kidney tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |