Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, ICC, IHC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2067-25UG |
| Description | Anti-Calnexin Antibody, clone AF8 |
| Alternate Names |
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| Background Information | Calnexin (UniProt: P27824; also known as IP90, Major histocompatibility complex class I antigen-binding protein p88, p90) is encoded by the CANX gene (Gene ID: 821) in human. Calnexin is a well-conserved, calcium binding, endoplasmic reticulum (ER) resident chaperone protein that associates with newly synthesized, incompletely folded monomeric glycoproteins and promotes their correct folding prior to secretion. It is also reported to target misfolded proteins for degradation. It is anchored to the ER membrane by a transmembrane domain. Calnexin associates with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes and may, therefore, function as a signaling complex regulating thymocyte maturation. Calnexin is synthesized with a signal peptide of 20 amino acids, which is cleaved to generate the mature form. Three isoforms of calnexin have been described that are generated by alternative splicing. Calnexin is shown to be phosphorylated at Ser564 by MAPK3/ERK1, which increases its association with ribosomes. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Calnexin, clone AF8, Cat. No. MABF2067, is a mouse monoclonal antibody that detects calnexin in human cells and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 1 µg from a representative lot detected Calnexin in one million MCF7 cells. Immunoprecipitation Analysis: A representative lot detected Calnexin in Immunoprecipitation applications (Hochstenbach, F., et. Al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8; Rajagopalan, S., et. Al. (1994). J Exp Med. 180(1):407-12). Immunohistochemistry Analysis: A representative lot detected Calnexin in Immunohistochemistry applications (Hochstenbach, F., et. Al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8). Flow Cytometry Analysis: A representative lot detected Calnexin in Flow Cytometry applications (Rajagopalan, S., et. Al. (1994). J Exp Med. 180(1):407-12). Western Blotting Analysis: A representative lot detected Calnexin in Western Blotting applications (Hochstenbach, F., et. Al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8). Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Calnexin in HeLa, A431 and Huvec cell lines. |
| Biological Information | |
|---|---|
| Immunogen | TCR complexes isolated from the human T-cell leukemia line, DND41. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone AF8 detects Calnexin in human cells. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 67.57 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in MCF7 cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Calnexin in MCF7 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |