Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IH(P), IP, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2071-25UG |
| Description | Anti-PLVAP Antibody, clone MECA-32 |
| Alternate Names |
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| Background Information | Plasmalemma vesicle-associated protein (UniProt: Q91VC4; also known as MECA-32 antigen, Plasmalemma vesicle protein 1, PV-1) is encoded by the Plvap (also known as Pv1) gene (Gene ID: 84094) in murine species. MECA-32 is a single-pass type II membrane protein that is involved in the formation of stomatal and fenestral diaphragms of caveolae and may also function in microvascular permeability. It is expressed in lung, kidney, spleen, heart, muscle, eye, pancreas, thyroid, thymus, submaxillary gland, prostate, epididymis, uterus, and liver. MECA-32 contains three coiled coil regions (aa 140-160; 189-224; and 281-383). It has a short cytoplasmic domain (aa 1-26), a transmembrane domain (aa 27-47), and a long extracellular domain (aa 48-438). In mouse embryo it is expressed at 7 days post coitum (dpc). Its expression in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier. Between 12 to 16 dpc higher expression is observed in endothelial cell surfaces of blood vessels in the brain. (Ref.: Hallmann, R., et Al. (1995). Dev. Dynamics. 202; 525-332). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PLVAP, clone MECA-32, Cat. No. MABF2071, is a rat monoclonal antibody tht detects Plasmalemma vesicle-associated protein (PV_1) and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot detected PLVAP in Immunohistochemistry applications (Tanneau, g.M., et. Al. (1999). J Histochem Cytochem. 47(12):1581-92; Hallmann, R., et. Al. (1995). Dev Dyn. 202(4):325-32). Immunohistochemistry Analysis: A representative lot detected PLVAP in Immunohistochemistry applications (Kopp, H.g., et. Al. (2005). Blood. 106(2):505-13). Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PLVAP in mouse liver tissue. Immunoprecipitation Analysis: A representative lot detected PLVAP in Immunoprecipitation applications (Hallmann, R., et. Al. (1995). Dev Dyn. 202(4):325-32). Western Blotting Analysis: A representative lot detected PLVAP in Western Blotting applications (Hallmann, R., et. Al. (1995). Dev Dyn. 202(4):325-32). |
| Biological Information | |
|---|---|
| Immunogen | Mouse lymph node stromal cells. |
| Clone | MECA-32 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone MECA-32 is a rat monoclonal antibody that detects Plasmalemma vesicle-associated protein (PLVAP) in murine species. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Predicted to react with Human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 50- 55 kDa observed; 49.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse kidney tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected PLVAP in mouse kidney tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |