Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Virus | FC, ELISA, NEUT, FACS | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2073-25UG |
| Description | Anti-Rabies Virus CVS-11 Antibody, clone 509-6 |
| Alternate Names |
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| Background Information | Glycoprotein (UniProt: O92284) is encoded by the g gene in Rabies virus, strain CVS-11. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. Clone 509-6 is shown to neutralize all fixed and street rabies virus strains except some virus isolates from bats. The conformational antigenic site I for this clone is located at aa 231. (Ref.: Wiktor, TJ and Koprowski, H (1980). J. Exp. Med. 152: 99-112; Marissen, WE et Al (2005). J. Virol. 79(8): 4672–4678). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Rabies Virus CVS-11 , clone 509-6 Antibody, Cat. No. MABF2073-25UG, validated for use in Flow Cytometry and ELISA, Neutralizing and Fluorescence Activated Cell Sorting (FACS). |
| Key Applications |
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| Application Notes | Neutralizing Analysis: A representative lot neutralized Rabies Virus (Schumacher, C.L., et. Al. (1989). J Clin Invest. 84(3):971-5; Bunschoten, H., et. Al. (1989). J Gen. Virol. 70 ( Pt 2):291-8; Marissen, W.E., et. Al. (2005). J Virol. 79(8):4672-8). |
| Biological Information | |
|---|---|
| Immunogen | Beta-propiolactone inactivated Rabies virus, strain CVS-11 (RABV). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 509-6 detected glycoprotein from Rabies virus, strain CVS-11 in L929 cells expressing the rabies protein. It did not detect glycoprotein in wild type L929 cells. |
| Isotype | IgG2a |
| Species Reactivity |
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| Species Reactivity Note | Rabies Virus. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in L929 cells expressing the rabies protein and in L929 (WT) cells. Flow Cytometry Analysis: 1 µg of this antibody detected glycoprotein of Rabies virus in one million L929 cells expressing the rabies protein. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |