Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Virus | IP, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2074-25UL |
| Description | Anti-MuLV TM Antibody, clone 42-114 |
| Alternate Names |
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| Background Information | The murine leukemia virus (MuLV) is a gammaretrovirus that has a positive, single-stranded sense RNA, which replicates via reverse transcription. The envelope of the virus is shown to be covered with glycoprotein spikes. Three major genes produced by the murine leukemia virus are gag, pol, and env. The gag gene codes for the group-specific antigen that is responsible for the production of the viral matrix capsid and nucleoproteins. The pol gene encodes reverse transcriptase, a protease, and an integrase. The reverse transcriptase is used to make complementary DNA by reverse transcribing its own RNA into DNA. The reverse transcriptase in MuLV is able to act as a monomer as opposed to a dimer. The integrase integrates the synthesized viral DNA into the host cell s DNA. The env gene codes for a glycosylated protein that is processed into the two viral envelope proteins, gp70 and p15(E), which aid the virus in selecting and attacking host cells. The gp70 protein has the receptor binding activity of the virus. Retrovirus membrane fusion is catalyzed by the Env, surface (SU) and transmembrane (TM) proteins. The SU protein is involved in receptor binding, while the TM protein contains the necessary elements for membrane fusion. Clone 42-114 is TM-specific and does not recognize G541R mutants. (Ref. Pinter,A., et Al. (1982). Virology, 116(2); 499-516. Schneider, WM., et Al. (2008). Virology. 371(1); 165-174). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgM in PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MuLV TM, clone 42-114, Cat. No. MABF2074, is a rat monoclonal antibody that detects Murine leukemia virus (MuLV) transmembrane protein and has been tested for use in Immunoprecipitation and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot immunoprecipitated MuLV TM (Pinter, A., et. Al. (1982). Virology. 116(2):499-516). Western Blotting Analysis: A representative lot detected MuLV TM in Western Blotting applications (Schneider, W.M., et. Al. (2008). Virology. 371(1):165-74). |
| Biological Information | |
|---|---|
| Immunogen | AKR K36 and AKR SL3 leukemia virus. |
| Clone | 42-114 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 42-114 detects murine leukemia virus in infected cells. |
| Isotype | IgMκ |
| Species Reactivity |
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| Species Reactivity Note | Murine Leukemia Virus. |
| Antibody Type | Monoclonal Antibody |
| Molecular Weight | ~15 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cells infected with murine leukemia virus (MuLVs). Western Blotting Analysis: A 1:250 dilution of this antibody detected MuLV TM in HEK293 cells infected with murine leukemia virus (MuLVs). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |