Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, ICC, IF, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2078-25UG |
| Description | Anti-APN/CD13 Antibody, clone DF-5 |
| Alternate Names |
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| Background Information | Aminopeptidase N (UniProt: P15144; also known as EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13) is encoded by the ANPEP (also known as APN, CD13, PEPN) gene (Gene ID: 290) in human. Aminopeptidase N is a single-pass type II membrane protein that is also found as a soluble form in cells. It is widely expressed as a homodimer of 280 kDa on the cell surface in many tissues, including intestinal epithelia and the nervous system. It serves as a broad specificity aminopeptidase that plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It preferentially cleaves N-terminus neutral amino acids, most notably alanine residue. Aminopeptidase N is involved in many physiological processes, including antigen presentation regulation, differentiation, proliferation, apoptosis, cancer metastasis, and angiogenesis. It is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Human Aminopeptidase N consists of a short N-terminal cytoplasmic end (aa 2-8), a transmembrane domain (aa 9-32), and a large extracellular portion (aa 33-967), which is composed of a Ser/Thr-rich region (aa 33-68) and the metalloprotease domain (aa 69-967). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-APN/CD13, clone DF-5, Cat. No. MABF2078, detects Aminopeptidase N and has been tested for use in Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in human prostate tissue. Flow Cytometry Analysis: A representative lot detected APN/CD13 in Flow Cytometry applications (Sorrell, J.M., et. Al. (2003). Exp Dematol. 12(3):315-23). Immunofluorescence Analysis: A representative lot detected APN/CD13 in Immunofluorescence applications (Sorrell, J.M., et. Al. (2003). Exp Dematol. 12(3):315-23; Sorrell, J.M., et. Al. (2007). Cell Tissue Res. 327(3):499-510). Western Blotting Analysis: A representative lot detected APN/CD13 in Western Blotting applications (Sorrell, J.M., et. Al. (2003). Exp Dematol. 12(3):315-23). Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in PC3 cell line. |
| Biological Information | |
|---|---|
| Immunogen | Crude cell membrane fraction from adult human papillary and reticular dermal fibroblasts. |
| Clone | DF-5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone DF-5 specifically detects aminopeptidase N in human cells. |
| Isotype | IgG3κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 109.54 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human kidney tissue. Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected APN/CD13 in human kidney tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |