Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, Function Assay | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2079 |
| Description | Anti-CD8 alpha Antibody, clone 53-6.72 |
| Alternate Names |
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| Background Information | T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the CD8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 alpha is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved to form the mature protein that has an extracellular domain (aa 28-196), a helical domain (aa 197-217), and a cytoplasmic tail (aa 218-247). CD8 is generally present as a heterodimer of alpha and beta chains linked by two disulfide bonds, however, it can also form homodimers. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 plays a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 acts as a co-receptor for the T cell receptor (TCR) and like the TCR, it binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rat monoclonal Anti-CD8 alpha, clone 53-6.72, Cat. No. MABF2079, is a rat monoclonal antibody that detects CD8 alpha and has been tested for use in Flow Cytometry and functional studies. |
| Key Applications |
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| Application Notes | Flow Cytometry Analysis: A representative lot detected CD8 alpha in Flow Cytometry applications (de Latour, R.P., et. Al. (2010). Blood. 116(20):4175-84). Functional Analysis: A representative lot detected CD8 alpha in Functional analysis applications (Daley, D., et. Al. (2016). Cell. 166(6):1485-1499; Hannani, D., et. Al. (2014). Cell Death Differ. 21(1):50-8). |
| Biological Information | |
|---|---|
| Immunogen | Murine Thymic/splenic lymphocytes. |
| Clone | 53-6.72 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 53-6.72 is a rat monoclonal antibody that detects CD8 alpha chain in murine thymocytes. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 27.46 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in mouse thymocytes. Flow Cytometry Analysis: 1 µg of this antibody detected CD8 alpha in one million mouse thymocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |