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Millipore This Rat Monoclonal Anti-Cd8 Alpha, Clone 53-6.72, Cat. No. Mabf2079, Is A Rat Monoclonal Antibody That Detects Cd8 Alpha And Has Been Tested For Use In Flow Cytometry And Functional Studies. - Mill (Additional S&H or Hazmat Fees May Apply)
T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the CD8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 alpha is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved to form the mature protein that has an extracellular domain (aa 28-196), a helical domain (aa 197-217), and a cytoplasmic tail (aa 218-247). CD8 is generally present as a heterodimer of alpha and beta chains linked by two disulfide bonds, however, it can also form homodimers. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 plays a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 acts as a co-receptor for the T cell receptor (TCR) and like the TCR, it binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets.
Product Information
Format
Purified
Presentation
Purified rat monoclonal antibody IgG2a in PBS without azide.
Applications
Application
This rat monoclonal Anti-CD8 alpha, clone 53-6.72, Cat. No. MABF2079, is a rat monoclonal antibody that detects CD8 alpha and has been tested for use in Flow Cytometry and functional studies.
Key Applications
Flow Cytometry
Inhibits Activity/Function
Application Notes
Flow Cytometry Analysis: A representative lot detected CD8 alpha in Flow Cytometry applications (de Latour, R.P., et. al. (2010). Blood. 116(20):4175-84).
Functional Analysis: A representative lot detected CD8 alpha in Functional analysis applications (Daley, D., et. al. (2016). Cell. 166(6):1485-1499; Hannani, D., et. al. (2014). Cell Death Differ. 21(1):50-8).
Biological Information
Immunogen
Murine Thymic/splenic lymphocytes.
Concentration
Please refer to lot specific datasheet.
Host
Rat
Specificity
Clone 53-6.72 is a rat monoclonal antibody that detects CD8 alpha chain in murine thymocytes.
Isotype
IgG2a
Species Reactivity
Mouse
Species Reactivity Note
Mouse.
Antibody Type
Monoclonal Antibody
Gene Symbol
CD8a
Lyt2
Lyt-2
Purification Method
Protein G purified
UniProt Number
P01731
Molecular Weight
27.5 kDa calculated.
Product Usage Statements
Quality Assurance
Evaluated by Flow Cytometry in mouse thymocytes.
Flow Cytometry Analysis: 1 µg of this antibody detected CD8 alpha in one million mouse thymocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
