Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, FC, ICC, INHIB, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2084-50UL |
| Description | Anti-Phosphorylcholine Antibody, clone BH8 |
| Alternate Names |
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| Background Information | Phosphorylcholine (PC) is considered as an important immunodominant determinant of pneumococcal teichoic acids. It is found in the cell walls of a variety of pathogenic and nonpathogenic microorganisms, including Streptococcus pneumoniae, lactobacillus, certain fungi, and nematodes. PC plays an important protective role in pneumococcal bacterial infection. It is also a major prerequisite for proinflammatory effects of PAF and PAF-like lipids.Antibodies to PC are naturally present in humans and their levels are reported to decline with age. Natural PC-specific antibodies are reported to constitute about 5 to 10% of the total IgM pool in humans. Antibodies to PC have been subdivided into two populations, Group I and II, based on their affinity for PC and p-nitrophenyl phosphorylcholine (NPPC). Group I anti-PCs bind both PC and NPCC whereas Group II require a phenyl group and bind only to NPPC. Anti-PC IgM, IgA, and IgG1 belong to the Group I and Anti-PC IgG2 is entirely made up of Group II antibodies. Anti-PC IgM has are reported to exhibit a negative correlation with atherosclerosis development in hypertensive individuals and low levels of anti-PC may be used to predict development of cardiovascular disease. (Ref.: Su, J., et Al. (2006). Atherosclerosis. 188(1):160-166; Sjoberg, BG et Al. (2009). Atherosclerosis. 203(2); 528-532). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgM in PBS without azide. |
| Applications | |
|---|---|
| Application | Anti-Phosphorylcholine, clone BH8, Cat. No. MABF2084, is a mouse monoclonal antibody that detects Phosphorylcholine and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Inhibition/Function studies, and Western Blotting. |
| Key Applications |
|
| Application Notes | ELISA Analysis: A representative lot detected Phosphorylcholine in ELISA applications (Patel, P.S., et. Al. (2015). J. Immunol. 194(12):5838-50). Inhibits Activity/Function Analysis: A representative lot inhibited lung-resident APC activation and diminished the infiltration of allergy-associated cells into the pulmonary parenchyma in mice. (Patel, P.S., et. Al. (2015). J Immunol. 194(12):5838-50). ELISA Analysis: A representative lot detected Phosphorylcholine in ELISA applications (Ansel, K.M., et. Al. (2002). Immunity. 16(1):67-76). Flow Cytometry Analysis: A representative lot detected Phosphorylcholine in Flow Cytometry applications (Patel, P.S., et. Al. (2015). J. Immunol. 194(12):5838-50). Immunocytochemistry Analysis: A representative lot detected Phosphorylcholine in Immunocytochemistry applications (Patel, P.S., et. Al. (2015). J. Immunol. 194(12):5838-50). |
| Biological Information | |
|---|---|
| Immunogen | Bacteria (Streptococcus pneumoniae, non-encapsulated strain R36A). |
| Clone | BH8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone BH8 speifically detects phosphocholine conjugated to BSA. |
| Isotype | IgMκ |
| Species Reactivity |
|
| Species Reactivity Note | All. |
| Antibody Type | Monoclonal Antibody |
| Molecular Weight | ~66 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Phosphorylcholine-BSA conjugate. Western Blotting Analysis: A 1:250 dilution of this antibody detected Phosphorylcholine-BSA conjugate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |