| Description | |
|---|---|
| Catalogue Number | MABF2087-25UL |
| Description | Anti-Adenylate Cyclase Toxin Antibody, B. pertussis Antibody, clone 9D4 |
| Alternate Names |
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| Background Information | Adenylate Cyclase Toxin (UniProt: C8C508; also known as ACT or Cya A) is one of several virulence factors produced by the bacterium Bordetella pertussis. Bordetella pertussis, a gram-negative bacterium, is shown to be the causative agent for whooping cough. It produces several virulence factors, including Cya A. The CyaA toxin is a bifunctional protein of 1,706 residues consisting of an NH2-terminal catalytic domain of 400 amino acids and a COOH-terminal part with 1,306 residues. The CyaA polypeptide is synthesized as an inactive protoxin, which is then converted to an active toxin by posttranslational palmitoylation of two internal lysine residues (Lys 856 and 963). Upon interaction of its C-terminal hemolysin moiety with the cell surface receptor alphaM-beta2 integrin ((Cd11b/Cd18), the N-terminal cyclase domain translocates into the host cell cytosol where it generates supraphysiological levels of cAMP concentration, which inhibits host cell anti-bacterial activities and apoptotic death of macrophages. Cya A toxin is also shown to elicit potassium ion efflux from erythrocytes in a process that is thought to be a precursor event to osmotic lysis of erythrocytes. Clone 9D4 binds to an epitope of AC toxin in the glycine- and aspartate- rich region. Hence, it recognizes other RTX toxins in addition to AC toxin. Clone 9D4 increases the fraction of AC toxin (CyaA) that is secreted to cell surface and release of CyaA into culture supernatant(Ref.: Guermonprez, P., et Al. (2001). J. Exp. Med. 193(9); 1035-1044; Gray, MC., et Al. (2001). J. Bacteriol. 183(20); 5904-5910; Holubova, J., et Al. (2012). Infect. Immun. 80(3); 1181-1192). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in PBS without preservatives. |
| Applications | |
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| Application | Anti-Adenylate Cyclase Toxin, B. pertussis, clone 9D4, Cat. No. MABF2087 is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis and has been tested for use in DOT Blot, Function Assay and Western Blotting. |
| Key Applications |
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| Application Notes | Function Analysis: A representative lot increased the fraction of AC toxin (CyaA) that is secreted to cell surface and release of CyaA into culture supernatant. (Gray, M.C., et. Al. (2001). J Bacteriol. 183(20):5904-10). Western Blotting Analysis: A representative lot detected Adenylate Cyclase Toxin, B. pertussis in Western Blotting applications (Holubova, J., et. Al. (2012). Infect Immun. 80(3):1181-92; Lee, S.J., et. Al. (1999). Infect Immun. 67(5):2090-5). DOT Blot Analysis: A representative lot detected Adenylate Cyclase Toxin, B. pertussis in DOT Blot applications (Lee, S.J., et. Al. (1999). Infect Immun. 67(5):2090-5). |
| Biological Information | |
|---|---|
| Immunogen | Adenylate cyclase toxin from Bordetella pertussis. |
| Clone | 9D4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 9D4 is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis. |
| Isotype | IgG2aλ |
| Species Reactivity |
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| Species Reactivity Note | Bacteria. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein g purified |
| Molecular Weight | ~220 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Adenylate cyclase toxin from Bordetella pertussis. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Adenylate Cyclase Toxin from from Bordetella pertussis. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |