Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, WB, IP, FUNC | SHm | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2089-25UG |
| Description | Anti-CD28 Antibody, clone 37.51 |
| Alternate Names |
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| Background Information | T-cell-specific surface glycoprotein CD28 (UniProt: P31041, also known as CD28) is encoded by the Cd28 gene (Gene ID: 12487) in murine species. CD28 is a single-pass type I membrane protein that exists as a disulfide linked homodimer. In human CD28 is expressed by a majority of T cells in the periphery, including all CD4+ cells and about 50% CD8+ cells. CD28 is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 2-150), a transmembrane domain (aa 151-177), and a cytoplasmic domain (aa 178-218). CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. It enhances the production of IL-4 and IL-10 in T-cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. B7-1 (CD80) and B7-2 (CD86) are two known ligands of CD28 on activated B cells and antigen presenting cells (APC). These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction co-stimulatory signaling between the B7 ligands and CD28 is essential for crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified syrian hamster monoclonal antibody IgG2-IgG3 in PBS without azide. |
| Applications | |
|---|---|
| Application | This syrian hamster monoclonal Anti-CD28, clone 37.51 Antibody, Cat. No. MABF2089 is tested for use in Flow Cytometry, Western Blotting, Immunoprecipitation, and Affects Function, for the detection of CD28. |
| Key Applications |
|
| Application Notes | Affects Function Analysis: A representative lot detected CD28 in Affects Function applications (Ulges, A., et. Al. (2015). Nat Immunol. 16(3):267-75; Krummel, M.F., et. Al. (1995). J Exp Med. 1;182(2):459-65; Brender, C., et. Al. (2007) Blood. 1;110(7):2528-36). Flow Cytometry Analysis: A representative lot detected CD28 in Flow Cytometry applications (Gross, J.A., et. Al. (1992). J Immunol. 149(2):380-8; Krummel, M.F., et. Al. (1995). J Exp Med. 1;182(2):459-65). Immunoprecipitation Analysis: A representative lot detected CD28 in Immunoprecipitation applications (Gross, J.A., et. Al. (1992). J Immunol. 149(2):380-8). Western Blotting Analysis: A representative lot detected CD28 in Western Blotting applications (Gross, J.A., et. Al. (1992). J Immunol. 149(2):380-8 |
| Biological Information | |
|---|---|
| Immunogen | EL-4 T lymphoma cells. |
| Clone | 37.51 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Syrian Hamster |
| Specificity | Clone 37.51 is a Syrian hamster monoclonal antibody that detects CD28 in murine cells. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 25.24 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in EL-4 T lymphoma cells. Flow Cytometry Analysis: 1 µg of this antibody detected CD28 in 1X10E6 EL-4 T lymphoma cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |