Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Virus | ELISA, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2097-25UG |
| Description | Anti-Norovirus (MNV-1) Antibody, clone 5C4.10 |
| Alternate Names |
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| Background Information | Norovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks. Murine norovirus (MNV-1) that is about 28 to 35 nm in diameter, shares many biochemical and genetic features with human noroviruses. An analysis of the MNV-1 genome shows three open reading frames (ORF) that are characteristic of noroviruses and vesiviruses. ORF1 is reported to encode a predicted 187 kDa polyprotein that contains the 2C helicase, 3C protease, and 3D polymerase motifs. ORF2 encodes a 59 kDa capsid protein that can self-assemble into virus-like particles when expressed in a baculovirus expression system. ORF3 is reported to encode a putative 22 kDa basic protein. The norovirus capsid protein contains a highly conserved shell (S) and protruding (P) domain and the P domain is shown to contain binding sites for receptors and neutralizing antibodies. The S domain is shown to be highly conserved and the capsid sequence diversity is limited to the P domain. MNV-1 infection is reported to modulate the MAPK pathway to activate eIF4E phosphorylation and the activation of p38 and Mnk during MNV-1 infection is considered to be important for its replication. This monoclonal antibody, clone 5C4.10, recognizes the S domain portion of the viral capsid protein and is broadly reactive with pan-noroviruses of murine and human type. (Ref.: Kolawole AO, et Al. (2017). mSphere 2: e00334-17; Kolawole, AO et Al. (2014). J. Gen. Virol. 95(9); , 1958 1968; Royall, E., et Al. (2015). J. Biol. Chem. 290(8); 4748-58; Wobus, CE et Al. (2006). J. Virol. 80(11); 5104-5112). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Norovirus (MNV-1), clone 5C4.10, Cat. No. MABF2097, is a mouse monoclonal antibody that detects murine Norovirus capsid protein and has been tested for use in ELISA and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Norovirus (MNV-1) in Western Blotting applications (Kolawole, A.O., et. Al. (2014). J Gen Virol. 95(Pt 9):1958-68). ELISA Analysis: A representative lot detected Norovirus (MNV-1) in ELISA applications (Kolawole, A.O., et. Al. (2014). J Gen Virol. 95(Pt 9):1958-68; Kolawole, A.O., et. Al. (2017). mSphere. 2(5). pii: e00334-17). |
| Biological Information | |
|---|---|
| Immunogen | Infectious and UV-inactivated murine norovirus-1. |
| Clone | 5C4.10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5C4.10 specifically recognizes the S domain portion of capsid protein of murine Norovisrus. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Norovirus. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein g purified |
| Molecular Weight | ~57 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in RAW 264.7 cells infected with Murine norovirus-1.CW3. Western Blotting Analysis: 1 µg/mL of this antibody detected Norovirus (MNV-1) in RAW 264.7 cells infected with Murine norovirus-1.CW3. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |