Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, FC, IP, Function Assay, Inhibition | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2105-25UG |
| Description | Anti-CD6 Antibody, clone UMCD6 |
| Alternate Names |
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| Background Information | T-cell differentiation antigen CD6 (UniProt: P30203; also known as T12, TP120, CD6) is encoded by the CD6 gene (Gene ID: 923) in human. CD6 is a single-pass type I membrane protein that serves as a cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166. It contributes to signaling cascades triggered by activation of the TCR/CD3 complex and functions as a costimulatory molecule to promote T-cell activation and proliferation. It is also involved in the formation and maturation of the immunological synapse. CD6 has been detected on peripheral blood T-cells and on natural killer (NK) cells and is also detected in spleen, thymus, appendix, lymph nodes. CD6 is known to bind both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria and LPS binding leads to the activation of signaling cascades and down-stream MAP kinases. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 18-402), a transmembrane domain (aa 403-423), and a cytoplasmic domain (aa 424-668). Seven isoforms of CD6 have been reported that are produced by alternative splicing. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without preservatives. |
| Applications | |
|---|---|
| Application | Anti-CD6, clone UMCD6, Cat. No. MABF2105 is a mouse monoclonal antibody that detects CD6 and has been tested for use in DOT Blot, Flow Cytometry, Immunoprecipitation, Function Analysis, Inhibition assay. |
| Key Applications |
|
| Application Notes | Inhibition Analysis: A representative lot inhibited antigen-specific responses to tetanus toxoid, but not to the superantigen SEA. (Singer, N.g., et. Al. (1996). Immunology. 88(4):537-43). Induces Function: A representative lot showed strong synergistic effect with phorbol ester in inducing T cell activation and enhanced the autologous mixed lymphocyte reaction. (Bott, C.M., et. Al. (1993). Int Immunol. 5(7):783-92). DOT Blot Analysis: A representative lot detected CD6 in DOT Blot applications (Bott, C.M., et. Al. (1993). Int Immunol. 5(7):783-92). Immunoprecipitation Analysis: A representative lot immunoprecipitated CD6 in Immunoprecipitation applications (Bott, C.M., et. Al. (1993). Int Immunol. 5(7):783-92). Flow Cytometry Analysis: A representative lot detected CD6 in Immunoprecipitation applications (Singer, N.g., et. Al. (1996). Immunology. 88(4):537-43; Bott, C.M., et. Al. (1993). Int Immunol. 5(7):783-92; Bott, C.M., et. Al. (1994). J Immunol. 153(1):1-9; Li, Y., et. Al. (2017). Proc Natl Acad Sci USA. 114(10):2687-2692). |
| Biological Information | |
|---|---|
| Immunogen | ST-1 T cells isolated from human knee synovium. |
| Clone | UMCD6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone UMCD6 specifically detects CD6 in human cells. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 71.80 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in PMA stimulated Jurkat cells. Flow Cytometry Analysis: A 1:1000 dilution of this antibody detected CD6 in one million Jurkat cells stimulated with PMA. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |