Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, FC, Inhibition | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2117-25UG |
| Description | Anti-insulin peptide–MHC Antibody, clone mAb287 |
| Background Information | Juvenile diabetes or insulin-dependent diabetes mellitus (IDDM) is considered as an autoimmune disease where islet infiltration of T cells, B cells, macrophages, and dendritic cells leads to their progressive destruction. It is reported that non-obese diabetic mice (NOD) express a single MHC class II molecule, IAg7, which is shown to be essential for the development of IDDM. It is shown that the major T cell insulin epitope resides with in amino acids 9-23 of the beta chain (B:9-23) and this peptide can bind to IAg7 in multiple positions (registers). Pathogenic CD4 T cells can recognize beta 9-23:I Ag7 complex when the insulin peptide is bound in register 3 (R3), which places amino acids 14-22 of the beta chain in the core P1 to P9 position and places arginine at P9 position, which is highly unfavored for the IAg7 pocket. The binding motifs of IAg7 are degenerate and contain small hydrophobic residues at P4 and P6 position. This monoclonal antibody (clone mAb287) selectively binds to B:9-23 when presented by IAg7 only in R3. It blocks the binding of IAg7-B:10-23 R3 tetramers to cognate T cells and impedes T cell responses to soluble B:9-23 peptides, but does not affect recognition of any other peptide bound to IAg7. This antibody is also shown to diminish T cell infiltration in NOD mice, delay the development of IDDM and overt hyperglycemia. (Ref.: Stratmann, T., et Al. (2000). J Immunol. 165(6); 3214-3225; Zhang, L., et Al. (2014). Proc. Natl. Acad. Sci. USA. 111 (7); 2656-2661). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without preservatives. |
| Applications | |
|---|---|
| Application | Anti-insulin peptide MHC, clone mAb287, Cat. No. MABF2117, is a mouse monoclonal antibody that detects Insulin peptide -MHC and has been tested for use in ELISA, Flow Cytometry, and Inhibition assay. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected insulin peptide MHC in Flow Cytometry applications (Zhang, L., et. Al. (2014). Proc Natl Acad Sci USA. 111(7):2656-61). ELISA Analysis: A representative lot detected insulin peptide MHC in ELISA applications (Zhang, L., et. Al. (2014). Proc Natl Acad Sci USA. 111(7):2656-61). Inhibition Analysis: A representative lot of this antibody inhibited in vitro responses of insulin-specific T-cell hybridomas/transfectomas. (Zhang, L., et. Al. (2014). Proc Natl Acad Sci USA. 111(7):2656-61). |
| Biological Information | |
|---|---|
| Immunogen | Soluble I-Ag7 bearing Insulin R3 peptides. |
| Clone | mAb287 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone mAb287 is a mouse monoclonal antibody that recognizes a molecular complex of mouse MHC class II (named I-Ag7) and a peptide derived from insulin B-chain, amino acids 9-22. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein g purified |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Isotype testing. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |