Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, ICC, IH(P), IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2119-25UG |
| Description | Anti-CD318 (CD6L) Antibody, clone 3A11 |
| Alternate Names |
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| Background Information | CUB domain-containing protein 1 (UniProt: Q9H5V8; also known as Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with Src kinases, CD318, CD6L) is encoded by the CDCP1 (also known as TRASK) gene (Gene ID:64866) in human. CD318 is a single-pass membrane protein (isoform 1) that serves as a ligand for CD6. It is highly expressed in mitotic cells and has low expression during interphase. It is detected in high levels in skeletal muscle and colon tissue and in lower levels in kidney, placenta, lungs, and small intestine. Its levels are shown to be up-regulated in several human cancer tissues. It is also expressed in high levels in synovial tissues where it participates in CD6-dependent adhesion of T cells to synovial fibroblasts. Presence of CD318 on cancer cells is associated with their aggressiveness and metastatic potential. Preincubation of cancer cells with clone 3A11 is shown to enhance killing of these cells and inhibits their survival. CD318 is shown to be chemoattractive to T cells and levels of soluble CD318 are increased in the synovial fluid from patients with rheumatoid arthritis and juvenile inflammatory arthritis. CD318 is synthesized with a signal peptide (aa 1-29), which is cleaved off in the mature form. It has an extracellular domain (aa 30-667), a transmembrane domain (aa 668-688), and a cytoplasmic domain (aa 689-836). Three isoforms of CD318 have been described that are produced by alternative splicing. (Ref.:Ruth, JH., et Al. (2021). JCI Insight. doi: 10.1172/jci.insight.145662. Enyindah-Asonye, g., et Al. (2017). Proc. Natl. Acad. Sci. USA 114(33); E6912-E6921). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-CD318 (CD6L), clone 3A11, Cat. No. MABF2119, is a mouse monoclonal antibody that detects CD318 (CD6L) and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. Al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, g., et. Al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921). Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, g., et. Al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921). Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. Al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, g., et. Al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921). Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. Al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, g., et. Al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921). |
| Biological Information | |
|---|---|
| Immunogen | HBL-100 human mammary gland epithelial cells. |
| Epitope | extracellular domain |
| Clone | 3A11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 92.93 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HaCaT epidermal keratinocyte cells. Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected CD318 (CD6L) in HaCaT epidermal keratinocyte cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |