Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2122-100UG |
| Description | Anti-PCBP2 (hnRNP E2) Antibody, clone PC81-1 |
| Alternate Names |
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| Background Information | Poly(RC)-binding protein 2 (UniProt: Q15366; also known as Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2) is encoded by the PCBP2 gene (Gene ID: 5094) in human. PCBP2 belongs to a class of cellular proteins that bind to poly(C) stretches of both RNA and DNA and has been detected in all tissues studied. PCBP2 shuttles between the nucleus and cytoplasm and exerts its impact on RNA processing and mRNA expression through sequence-specific interactions with C-rich determinants within target mRNAs. It acts as an important mediator of multiple processes, including replication of viruses with gastrointestinal and hepatic tropism, hepatic collagen synthesis, globin expression, and cellular proliferation. PCBP2 is shown to negatively regulate cellular antiviral responses mediated by mitochondrial antiviral-signaling protein (MAVS) signaling and acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, thereby triggering MAVS ubiquitination and degradation. Together with PCBP1, it is shown to function as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP2 contains three K-homologous (KH) domains that mediates poly(C) binding. Eight different isoforms of PCBP2 have been described that are produced by alternative splicing. (Ref.: Ghanem, LR et Al. (2014). Gene Exp. Patterns 14(2); 78-87; Sean, P., et Al. (2008). Virology. Virology. 378(2); 243-253). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-PCBP2 (hnRNP E2), clone PC81-1, Cat. No. MABF2122, is a mouse monoclonal antibody that detects Poly(RC)-binding protein 2 and has been tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. Al. (2008). Virology. 378(2):243-53; Chase, A.J., et. Al. (2014). Virology. 449:35-44). Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127). Western Blotting Analysis: 1 µg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full-length human recombinant Poly(RC)-binding protein 2. |
| Clone | PC 81-1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone PC 81-1 specifically detects Poly(RC)-binding protein 2 in human cells. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~38 kDa observed; 38.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.1 µg/mL of this antibody detected PCBP2 (hnRNP E2) in HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |