Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Bab, Ca, Rb | WB, ELISA, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2127-100UG |
| Description | Anti-Properdin Antibody, clone 3A3E1 |
| Alternate Names |
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| Background Information | Properdin (UniProt: P27918; also known as Complement factor P) is encoded by the CEP (also known as PFC) gene (Gene ID: 5199) in human. (ABF185: Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without preservatives. |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Properdin, clone 3A3E1 Antibody, Cat. No. MABF2127 is tested for use in Western Blotting, ELISA, Immunohistochemistry (Paraffin)for the detection of Properdin. Inhibitis the functionn of properdin by inhibiting its binding to C3b. |
| Key Applications |
|
| Application Notes | Inhibition Analysis: A representative lot detected Properdin in Inhibition applications (Blatt, A.Z., et. Al. (2016). J Immunol. 196(11):4671-4680). Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Properdin in human tonsil tissue sections. ELISA Analysis: A representative lot detected Properdin in ELISA applications (Blatt, A.Z., et. Al. (2016). J Immunol. 196(11):4671-4680). |
| Biological Information | |
|---|---|
| Immunogen | Purified properdin from human plasma. |
| Clone | 3A3E1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3A3E1 specifically detects human properdin. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Baboon, Canine, Rabbit, Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~51 kDa observed; 51.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in purified human properdin. Western Blotting Analysis: 1 µg/mL of this antibody detected Properdin in Purified human properdin. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |