Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ca, Rb, Bab | WB, ELISA, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF2128-100UG |
| Description | Anti-Properdin Antibody, clone 6E11A4 |
| Alternate Names |
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| Background Information | Properdin (UniProt: P27918; also known as Complement factor P) is encoded by the CEP (also known as PFC) gene (Gene ID: 5199) in human. (ABF185: Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without preservatives. |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Properdin, clone 6E11A4 Antibody, Cat. No. MABF2128 is tested for use in Western Blotting, ELISA, and Immunohistochemistry (Paraffin), for the detection of Properdin. Inhibits function in Properdin. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Properdin in human tonsil tissue sections. ELISA Analysis: A representative lot detected Properdin in ELISA applications (Blatt, A.Z., et. Al. (2016). J Immunol. 196(11):4671-4680). Inhibition Analysis: A representative lot detected Properdin in Inhibition applications (Blatt, A.Z., et. Al. (2016). J Immunol. 196(11):4671-4680). |
| Biological Information | |
|---|---|
| Immunogen | Purified properdin from human plasma. |
| Clone | 6E11A4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 6E11A4 specifically detects human properdin. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Canine, Rabbit, Human, Baboon. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~51 kDa observed; 51.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in purified human properdin. Western Blotting Analysis: 1 µg/mL of this antibody detected Properdin in purified human properdin. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |