| Description | |
|---|---|
| Catalogue Number | MABF2147-100UG |
| Description | Anti-Aminopeptidase N (CD13) Antibody, clone 452 |
| Alternate Names |
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| Background Information | Aminopeptidase N (UniProt: P15144; also known as EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13) is encoded by the ANPEP (also known as APN, CD13, PEPN) gene (Gene ID: 290) in human. Aminopeptidase N is a single-pass type II membrane protein that is also found as a soluble form in cells. It is widely expressed as a homodimer of 280 kDa on the cell surface in many tissues, including intestinal epithelia and the nervous system. It serves as a broad specificity aminopeptidase that plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It preferentially cleaves N-terminus neutral amino acids, most notably alanine residue. Aminopeptidase N is involved in many physiological processes, including antigen presentation regulation, differentiation, proliferation, apoptosis, cancer metastasis, and angiogenesis. It is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Human Aminopeptidase N consists of a short N-terminal cytoplasmic end (aa 2-8), a transmembrane domain (aa 9-32), and a large extracellular portion (aa 33-967), which is composed of a Ser/Thr-rich region (aa 33-68) and the metalloprotease domain (aa 69-967). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Applications | |
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| Application | Anti-Aminopeptidase N (CD13), clone 452, Cat. No. MABF2147, is a mouse monoclonal antibody that detects Aminopeptidase N and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist function, and Western Blotting. |
| Key Applications |
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| Application Notes | Immunocytochemistry Analysis: A representative lot detected Aminopeptidase N (CD13) in Immunocytochemistry applications (Rahman, M.M., et. Al. (2014). Front Physiol. 4:402; Subramani, J., et. Al. (2013). J Immunol. 191(7):3905-12). Flow Cytometry Analysis: A representative lot detected Aminopeptidase N (CD13) in Flow Cytometry applications (Subramani, J., et. Al. (2013). J Immunol. 191(7):3905-12). Western Blotting Analysis: A representative lot detected Aminopeptidase N (CD13) in Western Blotting applications (Rahman, M.M., et. Al. (2014). Stem Cells. 32(6):1564-77; Rahman, M.M., et. Al. (2014). Front Physiol. 4:402). Agonist Analysis: A representative lot induced a temporal increase in phosphorylation of FAK, ERK and Src kinases in U937 cells. (Subramani, J., et. Al. (2013). J Immunol. 191(7):3905-12). Immunoprecipitation Analysis: A representative lot immunoprecipitated Aminopeptidase N (CD13) in Immunoprecipitation applications (Subramani, J., et. Al. (2013). J Immunol. 191(7):3905-12). |
| Biological Information | |
|---|---|
| Immunogen | Isolated human dermal fibroblasts. |
| Clone | 452 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 452 specifically detects Aminopeptidase N (CD13) in human cells. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 109.54 kDa calculated. |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Flow Cytometry in U937 cells. Flow Cytometry Analysis: 1 µg of this antibody detected Aminopeptidase N (CD13) in one million U937 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |