| Description | |
|---|---|
| Catalogue Number | MABF2159-100UG |
| Description | Anti-CD63 (LAMP3) Antibody, clone ME491 |
| Alternate Names |
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| Background Information | CD63 antigen (UniProt: P08962; also known as Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63) is encoded by the CD63 (also known as MLA1, TSPAN30) gene (Gene ID: 967) in human. CD63 is a multi-pass membrane protein of the tetraspan family that is found on endosome, lysosome, and plasma membranes. CD63 has been detected in platelets, Dysplastic nevi benign moles), radial growth phase primary melanomas, hematopoietic cells, and in tissue macrophages. In melanoma cells it is involved in their motility and adhesion. CD63 also plays a role in the adhesion of leukocytes onto endothelial cells. It is reported to play a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases and promote cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration. CD63 is a highly N-glycosylated protein with three asparagine glycosylation sites (aa 130, 150, 172) and its ribophorin II (RPN2)-mediated glycosylation has been linked to breast cancer. Overexpression of CD63 has been observed in esophageal cancer that is negatively correlated with tumor stage and lymph node metastasis. Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS), an autosomal recessive disorder that is characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This antibody (clone ME491) is shown to react with human primary and to some extent with metastatic melanoma tissues. (Ref.: Lai, X., et al. (2017). Oncol. Let. 13(6); 4245-4251; Tominaga, N., et al. (2014). Mol. Cancer 13; 134; Smith, M., et al. (1997). Melanoma Res. 7 (Suppl. 2), 163-170). |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-CD63 (LAMP3), clone ME491, Cat. No. MABF2159, is a mouse monoclonal antibody that detects CD63 antigen and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
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| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD63 (LAMP3) in human spleen and human bone marrow tissue sections. Immunocytochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunocytochemistry applications (Atkinson, B., et. al. (1984). Cancer Res. 44(6):2577-81). Flow Cytometry Analysis: A representative lot detected CD63 (LAMP3) in Flow Cytometry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9). Western Blotting Analysis: A representative lot detected CD63 (LAMP3) in Western Blotting applications (Smith, M., et. al. (1997). Melanoma Res. 7 Suppl 2:S163-70). Immunohistochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunohistochemistry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9). |
| Biological Information | |
|---|---|
| Immunogen | Clear supernatant from SK-Mel-23 cell lysate. |
| Clone | ME491 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone ME491 specifically detects CD63 (LAMP-3) in human cells. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~43 kDa observed; 25.64 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in human platelet lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected CD63 (LAMP3) in human platelet lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 μg |