| Description | |
|---|---|
| Catalogue Number | MABF2169-25UG |
| Description | Anti-HLA-g Antibody, clone 4H84 |
| Alternate Names |
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| Background Information | HLA class I histocompatibility antigen, alpha chain g (UniProt: P17693; also known as HLA g antigen, MHC class I antigen g) is encoded by the HLA-g (also known as HLA-6.0, HLAG) gene (Gene ID: 3135) in human. HLA-g is a single-pass type I membrane glycoprotein that serves as marker of extravillous cytotrophoblasts in placenta and plays a central role in molecule maternal tolerance of the semiallogeneic fetus. It is involved in the presentation of foreign antigens to the immune system. HLA-g is a heterodimer of an alpha chain and a beta chain. Severn different isoforms of HLA-g have been described that are produced by alternative splicing and their distribution is temporally affected during pregnancy in placenta and fetal tissues. HLA-g expression has been reported in renal cell carcinoma where it is exclusively restricted to tumor cells. Its expression is thought to be involved in the impairment of the anti-tumor immunity or resistance to immunotherapeutic agents. HLA-g is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form, which contains an extracellular domain (aa 25-308), a transmembrane domain (aa 309-332), and a short cytoplasmic tail (aa 333-338). |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-HLA-g, clone 4H84, Cat. No. MABF2169, is a mouse monoclonal antibody that detects HLA class I histocompatibility antigen, alpha chain g and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
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| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected HLA-g in human spleen tissue sections. Immunohistochemistry (Paraffin) Analysis: A representative lot detected HLA-g in Immunohistochemistry applications (Tabata, T., et. Al. (2012). Am J Pathol. 181(5):1540-59; Menier, C., et. Al. (2003). Hum Immunol. 64(3):315-26; Ibrahim, E.C., et. Al. (2003). Am J Pathol. 162(2):501-8; Tabata, T., et. Al. (2016). Cell Host Microbe. 20(2):155-66; McMaster, M., et. Al. (1998). J Immunol. 160(12):5922-8). Western Blotting Analysis: A representative lot detected HLA-g in Western Blotting applications (Paul, P., et. Al. (1999). Cancer Res. 59(8):1954-60; McMaster, M., et. Al. (1998). J Immunol. 160(12):5922-8). ELISA Analysis: A representative lot detected HLA-g in ELISA applications (McMaster, M., et. Al. (1998). J Immunol. 160(12):5922-8). Flow Cytometry Analysis: A representative lot detected HLA-g in Flow Cytometry applications (Menier, C., et. Al. (2003). Hum Immunol. 64(3):315-26). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of Human MHC class I antigen g. The immunogen sequence is conserved in all isoforms. |
| Clone | 4H84 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4H84 specifically detects human HLA-g. It recognizes an epitope with in 23 amino acids from the alpha1 region of all HLA-g isoforms. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 38.22 kDa calculated. |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HLA-g in human placenta tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |