| Description | |
|---|---|
| Catalogue Number | MABF2182-25UL |
| Description | Anti-CD46 Antibody, clone TRA-2-10 |
| Alternate Names |
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| Background Information | Membrane cofactor protein (UniProt: P15529; also known as TLX, Trophoblast leukocyte common antigen, CD46) is encoded by the CD46 (also known as MCP; MIC10) gene (Gene ID: 4179) in human. CD46 is a single-pass type I membrane protein that is expressed by all cells except erythrocytes. It acts as a cofactor for complement factor I, a serine protease that protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. CD46 is synthesized with a signal peptide (aa 1-34), which is cleaved off in the mature form. The mature form contains an extracellular domain (aa 35-343), a transmembrane domain (aa 344-366), and a cytoplasmic domain (aa 367-392). It also contains four Sushi domains where Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles Virus H protein, domains 2 and 3 are essential for Herpes virus 6 binding, and domains 3 and 4 are required for interaction with Streptococcus Pyrogens M protein and are the most important for interaction with C3b and C4b. CD46 can be glycosylated on Asn 83, 114, and 273 and N-glycosylation on Asn 114 and 273 are required for its cytoprotective function. N-glycosylation on Asn 114 is also required for Measles virus binding and N-glycosylation on Asn-273 is required for Neisseria binding. CD46 can also be extensively O-glycosylated in the Ser/Thr-rich domain, which is required for Neisseria binding, but not for Measles virus binding. Mutations in CD46 gene have been linked to hemolytic uremic syndrome atypical 2 that has been characterized by microangiopathic hemolytic anemia, thrombocytopenia, and renal failure. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without preservatives. |
| Applications | |
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| Application | Anti-CD46, clone TRA-2-10, Cat. No. MABF2182, is a mouse monoclonal antibody that detects Membrane cofactor protein (CD46) and has been for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Inhibition assay. |
| Key Applications |
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| Application Notes | Inhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. Al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7). Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. Al. (2001). Clin Exp Immunol. 124(2):180-9). Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. Al. (1985). Ann Hum Genet. 49(Pt 1):31-9). Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. Al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7). |
| Biological Information | |
|---|---|
| Immunogen | 2102Ep human embryonal carcinoma cells. |
| Clone | TRA-2-10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone TRA-2-10 specifically detects CD46 in human cells. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 43.75 kDa calculated. |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Flow Cytometry in HeLa cells. Flow Cytometry Analysis: 1 µg of this antibody detected CD46 in one million HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
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| Material Size | 25 µL |