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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, FC, IHC, ICC, INHIB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF230 |
| Description | Anti-TIM-3, clone RMT3-23, Azide Free Antibody |
| Alternate Names |
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| Background Information | The protein called Hepatitis A virus cellular receptor 2 (HAVcr-20), T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3), T-cell immunoglobulin mucin receptor 3 (TIM-3), or T-cell membrane protein 3 and encoded by the human gene HAVCR2/TIM3/TIMD3 is an important protein in the regulation of macrophage activation. TIM-3 is also critical for immune tolerance as it inhibits T-helper type 1 (Th1) mediated auto and alloimmune responses. Finally TIM-3 plays an important role in T-cell homing in the thymus and lymph and is the receptor for the Galectin-9 an important protein modulating cell-cell and cell matrix interactions. TIM-3 is a membrane bound protein that is expressed largely by immune system cells. High TIM-3 expression is also associated with many tumors including renal cancer and acute myelogenous leukemia (AML), and its expression is also a target for anti-cancer therapy as anti-TIM-3 antibodies have been shown to eradicate leukemic stem cells (LSCs) in animal models of AML. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-TIM-3 antibody, clone RMT3-23, Azide Free is validated for use in western blotting, flow cytometry, IHC, ICC & Inhibits activity/Function for the detection of TIM-3. |
| Key Applications |
|
| Application Notes | Inhibits Activity/Function Assay: Co-administration of a representative lot by an independent laboraotory with FVAX significantly suppressed tumor growth to a greater extent than FVAX alone. Treatment with Anti-TIM-3 , clone RMT3-23, Azide Free also augmented antitumor activities of plasmid DNA encoding melanoma target gp100 or TRP2, suggesting that targeting TIM-3 or TIM-4 improves antitumor activiites of other forms of cancer vaccines. (Bagdadi, M., et al. (2013). Cancer Immunol Immunother. 62(4):629-637.). Flow Cytometry Analysis: 1 µg from a representative lot detected TIM-3 in TIM-3 transfected 1X10E6 BALB/L5178Y cells. Flow Cytometry Analysis: A representative lot detected TIM-3 in dendritic cells, macrophages, splenocytes, and hepatic mononuclear cells from acute graft-vs-host disease (aGVHD) mice (Oikawa, T., et al. (2006). J Immunol. 177(7):4281-4287.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected TIM-3 in liver tissue from normal and aGVHD mice (Oikawa, T., et al. (2006). J Immunol. 177(7):4281-4287.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected TIM-3 in HMGB1-deficient MEF cells (Chiba, S., et al. (2012). Nat Immunol. 13(9):832-842.). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to mouse TIM-3. |
| Clone | RMT3-23 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~31 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in TIM-3 transfected L5178Y cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected TIM-3 in 10 µg of TIM-3 transfected L5178Y cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |