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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IHC, FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF243 |
| Description | Anti-Par-2 Antibody, clone SAM11 |
| Alternate Names |
|
| Background Information | The protein named Proteinase-activated receptor 2 (PAR2/PAR-2), or Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like-1 and encoded by the human gene F2RL1/GPR11/PAR2 is a receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Also as part of the innate and adaptive immunity response, PAR2 acts as sensor for proteolytic enzymes generated during infection and works to promote inflammation and the inflammatory response. PAR2 is also involved in vascular and airway tone and in blood pressure regulation and many other G protein coupled receptor response pathways. PAR2 is a multi-pass membrane protein that is widely expressed in most tissues except brain or skeletal muscle. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Par-2 Antibody, clone SAM11 is an antibody against Par-2 for use in western blotting, IHC & flow cytometry. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Par-2 in human small intestine, coronary artery and aorta sections, and in human artery and vein tissues (Molino, M., et al. (1998). Arterioschler Throm Vasc Biol. 18(5):825-832.). Flow Cytometry Analysis: Arepresentative lot from an independent laboratory detected Par-2 in Par-2 transfected COS-1 cells (Molino, M., et al. (1997). J Biol Chem. 272(17):11133-11141.). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to the N-terminus of human Par-2. |
| Epitope | N-terminus |
| Clone | SAM11 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed. The calculated MW of this protein is 44 kDa. However, it may be observed at higher molecular weights due to glycosylation. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH/3T3 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Par-2 in 10 µg of NIH/3T3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |