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Millipore Anti-S100a9 Antibody, Clone 2a5 (Azide Free) Is An Antibody Against S100a9 For Use In Western Blotting & Activity Assay. - Mill (Additional S&H or Hazmat Fees May Apply)
S100A9, also known as Calgranulin-B, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, or S100 calcium-binding protein A9, and encoded by the gene S100A9/CAGB/MRP14/S100-A9 is a calcium and zinc binding protein that plays a prominent role in the regulation of inflammatory processes and immune responses. S100-A9 can induce neutrophil chemotaxis and adhesion. S100-A9 one of the subunits of Calprotectin, which is a complex of S100-A8 and S100-A9 proteins and is also critical for immune responses. S100-A9 is a cytoplasmic and secreted protein predominantly expressed in myeloid cells as well as neutrophils and monocytes but not expressed in lymphocytes. S100-A9 is found in high concentrations at sites of inflammation or in the serum of patients with inflammatory diseases. S100-A9 is also a cancer marker as it is up regulated in many cancers including gastric, esophageal, colon, breast and many more.
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgG1 in buffer containing PBS without preservatives.
Quality Level
MQ100
Applications
Application
Anti-S100A9 Antibody, clone 2A5 (Azide Free) is an antibody against S100A9 for use in western blotting & activity assay.
Key Applications
Western Blotting
Activity Assay
Application Notes
Activity Assay Analysis: A representative lot detected S100A9 and found that it increases neutrophil migration & induces the secretion of cytokines by monocytes (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e45478 1-12). Western Blotting Analysis: A representative lot detected S100A9 in mouse neutrophils and recombinant mS100A9 (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e45478 1-12).
Biological Information
Immunogen
Recombinant protein corresponding to mouse S100A9.
Clone
2A5
Concentration
1 mg/mL
Host
Rat
Isotype
IgG1
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_00126878
Gene Symbol
S100a9
Cagb
Mrp14
Purification Method
Protein G Purified
UniProt Number
P31725
Molecular Weight
~15 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected S100A9 in 10 µg of Raji cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
