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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF299 |
| Description | Anti-CCR2 Antibody, clone 8C3.1 |
| Alternate Names |
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| Background Information | C-C chemokine receptor type 2 (UniProt P41597; also known as C-C CKR-2, CC-CKR-2, CCR-2, CCR2, CD192, MCP-1-R, Monocyte chemoattractant protein 1 receptor) is encoded by the CCR2 (also known as CMKBR2) gene (Gene ID 729230) in human. CC chemokine receptors (CCRs) are 7-transmembrane G protein-coupled receptors (GPCRs) that bind CC chemokines (CCLs) and mediate their biological activities. CCRs and CCLs play a central role in physiological and pathological recruitment of immune cells. CCR2 can interact with CCL2, CCL8 and CCL16, it is found on the surface of monocytes, activated memory T cells, B cells, and basophils in humans, and also in peritoneal macrophages in mice. CCR2 plays an important role in the recruitment of monocytes/macrophages and T cells. CCR2-knockout mice show a partial defect in macrophage function, decreased Th1 immune response, reduced delayed-type hypersensitivity reaction, and are more susceptible to pathogenic bacteria infections. Human CCR2 is a 7-transmembrane (a.a. 43-70, 81-100, 115-136, 154-178, 207-226, 244-268, 286-309) GPCR with an N-terminal extracellular domain (a.a. 1-42) and a C-terminoal cytoplasmic domain (a.a. 310-374). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-CCR2 Antibody, clone 8C3.1, Cat. No. MABF299 is validated for use in Flow Cytometry and Western Blotting for the detection of CCR2. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 2 µL from a representative lot detected surface CCR2 expression among the gated lymphocytes population from one million human PMBCs. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human N-terminal extracellular domain. |
| Clone | 8C3.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 8C3.1 targets the N-terminal extracelluar domain of human CCR2 spliced isoforms 1&2 reported by Uniprot (P41597). |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~40-50 kDa observed. 41.92 kDa (isoform 1) and 41.06 kDa (isoform 2) calculated. Larger-than-calculated sizes and smeary banding pattern are consistent with the detection of differentially glycosylated CCR2 species. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in U937 cell lysate. Western Blotting Analysis (WB): A 100,000 dilution of this antibody detected CCR2 in 10 µg of human lymphoma U937 monocyte lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |