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Millipore This Rat Monoclonal Antibody Anti-Cd117 (C-Kit) (Mouse), Pe, Clone Ack2 Validated For Use In Ihc, Icc & Flow Cytometry For The Detection Of Cd117 (C-Kit). - Mill (Additional S&H or Hazmat Fees May Apply)
The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo. In addition, the antibody is reported to be cross-reactive with rat c-Kit and is extensively published for use in this species.
Product Information
Format
PE
Presentation
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This rat monoclonal antibody Anti-CD117 (c-kit) (mouse), PE, clone ACK2 validated for use in IHC, ICC & Flow Cytometry for the detection of CD117 (c-kit).
Key Applications
Immunohistochemistry
Immunocytochemistry
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
ACK2
Concentration
0.2 mg/mL
Host
Rat
Isotype
IgG2bκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
Kit
SI
Purification Method
Size Exclusion Chromatography
UniProt Number
P05532
Product Usage Statements
Quality Assurance
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.06 µg of this antibody detected CD117 (c-kit) in one million C57Bl/6 bone marrow cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
