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Millipore This Rat Monoclonal Antibody Anti-Cd25 (Mouse), Apc & Clone Pc61.5 Validated For Use In Ip;Ihc;Flow Cytometry For The Detection Of Cd25. - Mill (Additional S&H or Hazmat Fees May Apply)
The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.
Product Information
Format
APC
Presentation
Purified rat monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This rat monoclonal antibody Anti-CD25 (mouse), APC & clone PC61.5 validated for use in IP;IHC;Flow Cytometry for the detection of CD25.
Key Applications
Immunoprecipitation
Immunohistochemistry
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
PC61.5
Concentration
0.2 mg/mL
Host
Rat
Isotype
IgG1λ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
Il2ra
Il2r
Purification Method
Size Exclusion Chromatography
UniProt Number
P01590
Product Usage Statements
Quality Assurance
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 µg of this antibody detected CD25 in one million C57BI/6 bone marrow cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
