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Millipore This Mouse Monoclonal Antibody Anti-Cd4 (Human), Percp-Cy5.5, Clone Okt4 Validated For Use In Western Blotting, Ip, Icc, Immunofluorescence & Flow Cytometry For The Detection Of Cd4. - Mill (Additional S&H or Hazmat Fees May Apply)
The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The OKT4 antibody is widely used as a phenotypic marker for CD4 expression. It is cross-reactive with CD4 in several non-human species, including Chimpanzee, Cynomolgus and Rhesus. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone RPA-T4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065)
Product Information
Format
PerCP-Cy5.5
Presentation
Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This mouse monoclonal antibody Anti-CD4 (human), PerCP-Cy5.5, clone OKT4 validated for use in western blotting, IP, ICC, Immunofluorescence & Flow Cytometry for the detection of CD4.
Key Applications
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Western Blotting
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
OKT4
Concentration
5 µL (0.25 µg)/test
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
CD4
Purification Method
Size Exclusion Chromatography
UniProt Number
P01730
Product Usage Statements
Quality Assurance
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.25 µg of this antibody detected CD4 in one million human peripheral blood lymphocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
