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Millipore This Anti-Camk4, Clone 8c5b8 Antibody Is Validated For Use In Western Blotting, Ihc, Immunofluorescence & Elisa For The Detection Of Camk4. - Mill (Additional S&H or Hazmat Fees May Apply)
Calcium/calmodulin-dependent protein kinase type IV
CaMK IV
CAMK4
Background Information
Calcium/calmodulin-dependent protein kinase type IV (CaMK IV), also known as CaM kinase-GR, and encoded by the gene name CAMK4 or CAMK or CAMK-GR or CAMKIV, is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. CAMK4 is localized in hippocampal neuron nuclei and is associated with chromatin and the nuclear matrix in spermatids. Expression in CAMK4 can be found in brain, thymus, CD4 T-cells, testis, and epithelial ovarian cancer tissue. CAMK4 can be phosphorylated by CaMKK1 and CaMKK2 on Thr200 and dephosphorylated by protein phosphatase 2A.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-CAMK4, clone 8C5B8 Antibody is validated for use in western blotting, IHC, immunofluorescence & ELISA for the detection of CAMK4.
Key Applications
Western Blotting
Immunohistochemistry
Immunofluorescence
ELISA
Application Notes
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CAMK4 in ovarian and rectum cancer tissues.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected CAMK4 in HepG2 cells.
ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
Immunogen
Purified recombinant fragment of human CAMK4 expressed in E. Coli
Clone
8C5B8
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
CAMK4
CAMK
CAMK-GR
CAMKIV
Purification Method
Protein G Purified
UniProt Number
Q16566
Molecular Weight
~57 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in SK-N-SH, Raji and HeLa cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CAMK4 in SK-N-SH, Raji and HeLa cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
